Construction of recombinant adenovirus-5 vector to prevent replication-competent adenovirus occurrence
Human adenovirus-5 (hAd5) is an important gene delivery vector, which has been widely used in various fields of biomedicine, such as gene therapy, cancer therapy, and vaccine development. However, replication-competent adenovirus (RCA) generated when adenoviral vectors are prepared in HEK293 cells h...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-12-01
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| Series: | Acta Virologica |
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| Online Access: | https://www.frontierspartnerships.org/articles/10.3389/av.2023.11642/full |
| _version_ | 1827580270037958656 |
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| author | Wenbo Xie Yifei Yuan Bo Liu Min Liang Min Liang |
| author_facet | Wenbo Xie Yifei Yuan Bo Liu Min Liang Min Liang |
| author_sort | Wenbo Xie |
| collection | DOAJ |
| description | Human adenovirus-5 (hAd5) is an important gene delivery vector, which has been widely used in various fields of biomedicine, such as gene therapy, cancer therapy, and vaccine development. However, replication-competent adenovirus (RCA) generated when adenoviral vectors are prepared in HEK293 cells has remained a concern. In this study, the human adenovirus-5 was modified to shorten the length of homologous sequence between the adenovirus and HEK293 genomic DNA, thereby reducing the production of RCA. The recombinant hAd5 was amplified and serially passaged 12 times in HEK293 cells. The amounts of RCA at passage 2, 4, 6, 8, 10, and 12 were detected by quantitative real-time PCR. The results demonstrated that the modification of adenoviral vector could effectively reduce the production of RCA during serial passages in HEK293 cells. |
| first_indexed | 2024-03-08T22:15:37Z |
| format | Article |
| id | doaj.art-cc118301573c48b6a889173daa3501b0 |
| institution | Directory Open Access Journal |
| issn | 1336-2305 |
| language | English |
| last_indexed | 2024-03-08T22:15:37Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Acta Virologica |
| spelling | doaj.art-cc118301573c48b6a889173daa3501b02023-12-19T04:11:35ZengFrontiers Media S.A.Acta Virologica1336-23052023-12-016710.3389/av.2023.1164211642Construction of recombinant adenovirus-5 vector to prevent replication-competent adenovirus occurrenceWenbo Xie0Yifei Yuan1Bo Liu2Min Liang3Min Liang4School of Life Science, Shanghai University, Shanghai, ChinaGeneSail Biotech (Shanghai) Co., Ltd., Shanghai, ChinaGeneSail Biotech (Shanghai) Co., Ltd., Shanghai, ChinaSchool of Life Science, Shanghai University, Shanghai, ChinaGeneSail Biotech (Shanghai) Co., Ltd., Shanghai, ChinaHuman adenovirus-5 (hAd5) is an important gene delivery vector, which has been widely used in various fields of biomedicine, such as gene therapy, cancer therapy, and vaccine development. However, replication-competent adenovirus (RCA) generated when adenoviral vectors are prepared in HEK293 cells has remained a concern. In this study, the human adenovirus-5 was modified to shorten the length of homologous sequence between the adenovirus and HEK293 genomic DNA, thereby reducing the production of RCA. The recombinant hAd5 was amplified and serially passaged 12 times in HEK293 cells. The amounts of RCA at passage 2, 4, 6, 8, 10, and 12 were detected by quantitative real-time PCR. The results demonstrated that the modification of adenoviral vector could effectively reduce the production of RCA during serial passages in HEK293 cells.https://www.frontierspartnerships.org/articles/10.3389/av.2023.11642/fullreplication-competent adenovirusHEK293human adenovirus-5recombinant adenovirushomologous sequence |
| spellingShingle | Wenbo Xie Yifei Yuan Bo Liu Min Liang Min Liang Construction of recombinant adenovirus-5 vector to prevent replication-competent adenovirus occurrence Acta Virologica replication-competent adenovirus HEK293 human adenovirus-5 recombinant adenovirus homologous sequence |
| title | Construction of recombinant adenovirus-5 vector to prevent replication-competent adenovirus occurrence |
| title_full | Construction of recombinant adenovirus-5 vector to prevent replication-competent adenovirus occurrence |
| title_fullStr | Construction of recombinant adenovirus-5 vector to prevent replication-competent adenovirus occurrence |
| title_full_unstemmed | Construction of recombinant adenovirus-5 vector to prevent replication-competent adenovirus occurrence |
| title_short | Construction of recombinant adenovirus-5 vector to prevent replication-competent adenovirus occurrence |
| title_sort | construction of recombinant adenovirus 5 vector to prevent replication competent adenovirus occurrence |
| topic | replication-competent adenovirus HEK293 human adenovirus-5 recombinant adenovirus homologous sequence |
| url | https://www.frontierspartnerships.org/articles/10.3389/av.2023.11642/full |
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