Dietary Exposure of Pacific Oyster (Crassostrea gigas) Larvae to Compromised Microalgae Results in Impaired Fitness and Microbiome Shift
The Pacific oyster Crassostrea gigas is the world’s most cultivated oyster and seed supply is heavily reliant on hatchery production where recurring mass mortality events are a major constraint. Outbreaks of bacterial infection via microalgal feed are frequently implicated in these mortalities. This...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.706214/full |
| _version_ | 1818612491856379904 |
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| author | Julien Vignier Olivier Laroche Anne Rolton Pandora Wadsworth Karthiga Kumanan Branwen Trochel Xavier Pochon Xavier Pochon Nick King |
| author_facet | Julien Vignier Olivier Laroche Anne Rolton Pandora Wadsworth Karthiga Kumanan Branwen Trochel Xavier Pochon Xavier Pochon Nick King |
| author_sort | Julien Vignier |
| collection | DOAJ |
| description | The Pacific oyster Crassostrea gigas is the world’s most cultivated oyster and seed supply is heavily reliant on hatchery production where recurring mass mortality events are a major constraint. Outbreaks of bacterial infection via microalgal feed are frequently implicated in these mortalities. This study assessed the effects of feeding compromised microalgae to developing oyster larvae. Intentionally ‘stressed’ (high pH) or non-stressed microalgae were fed to 11 day-old oyster larvae at two feeding rations for 96 h, followed by a recovery period. Biological endpoints of larval performance were measured following the 96 h exposure and subsequent recovery. Bacterial communities associated with the microalgae feed, rearing seawater, and the oyster larvae, were characterized and correlated with effects on oyster fitness parameters. Feeding stressed algae to oyster larvae for 96 h increased the occurrence of deformities (>70% vs. 20% in control), reduced feeding and swimming ability, and slowed development. Following the recovery period, fewer larvae reached pediveliger stage (2.7% vs. 36% in control) and became spat (1.5% vs. 6.6% in control). The quantity of stressed algae supplied to oyster larvae also influenced overall larval performance, with high feeding rations generally causing greater impairment than low rations. Bacterial profiling using 16S rRNA showed that most bacterial families characterized in larval tissue were also present in larval rearing seawater and in the microalgae feed (98%). The rearing seawater showed the highest bacterial richness compared to the larval and the microalgal compartments, regardless of feeding regime. In larval tissue, bacterial richness was highest in stressed and high-feed treatments, and negatively correlated with larval fitness parameters. These results suggest significant dysbiosis induced by compromised feed and/or increased feed ration. Several bacterial genera (e.g., Halomonas, Marinomonas) were strongly associated with impaired larval performance while the presence of genera in larvae including Vibrio was closely associated with overfeeding. Our research demonstrated that metabarcoding can be effectively used to identify microbiota features associated with larval fitness. |
| first_indexed | 2024-12-16T15:47:04Z |
| format | Article |
| id | doaj.art-cc1ac291034b4a00b7a1a0f5cd3c7c25 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-16T15:47:04Z |
| publishDate | 2021-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-cc1ac291034b4a00b7a1a0f5cd3c7c252022-12-21T22:25:46ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-08-011210.3389/fmicb.2021.706214706214Dietary Exposure of Pacific Oyster (Crassostrea gigas) Larvae to Compromised Microalgae Results in Impaired Fitness and Microbiome ShiftJulien Vignier0Olivier Laroche1Anne Rolton2Pandora Wadsworth3Karthiga Kumanan4Branwen Trochel5Xavier Pochon6Xavier Pochon7Nick King8Aquaculture Group, Cawthron Institute, Nelson, New ZealandCoastal and Freshwater Group, Cawthron Institute, Nelson, New ZealandAquaculture Group, Cawthron Institute, Nelson, New ZealandMoana New Zealand Ltd., Nelson, New ZealandAquaculture Group, Cawthron Institute, Nelson, New ZealandCoastal and Freshwater Group, Cawthron Institute, Nelson, New ZealandCoastal and Freshwater Group, Cawthron Institute, Nelson, New ZealandInstitute of Marine Science, The University of Auckland, Warkworth, New ZealandAquaculture Group, Cawthron Institute, Nelson, New ZealandThe Pacific oyster Crassostrea gigas is the world’s most cultivated oyster and seed supply is heavily reliant on hatchery production where recurring mass mortality events are a major constraint. Outbreaks of bacterial infection via microalgal feed are frequently implicated in these mortalities. This study assessed the effects of feeding compromised microalgae to developing oyster larvae. Intentionally ‘stressed’ (high pH) or non-stressed microalgae were fed to 11 day-old oyster larvae at two feeding rations for 96 h, followed by a recovery period. Biological endpoints of larval performance were measured following the 96 h exposure and subsequent recovery. Bacterial communities associated with the microalgae feed, rearing seawater, and the oyster larvae, were characterized and correlated with effects on oyster fitness parameters. Feeding stressed algae to oyster larvae for 96 h increased the occurrence of deformities (>70% vs. 20% in control), reduced feeding and swimming ability, and slowed development. Following the recovery period, fewer larvae reached pediveliger stage (2.7% vs. 36% in control) and became spat (1.5% vs. 6.6% in control). The quantity of stressed algae supplied to oyster larvae also influenced overall larval performance, with high feeding rations generally causing greater impairment than low rations. Bacterial profiling using 16S rRNA showed that most bacterial families characterized in larval tissue were also present in larval rearing seawater and in the microalgae feed (98%). The rearing seawater showed the highest bacterial richness compared to the larval and the microalgal compartments, regardless of feeding regime. In larval tissue, bacterial richness was highest in stressed and high-feed treatments, and negatively correlated with larval fitness parameters. These results suggest significant dysbiosis induced by compromised feed and/or increased feed ration. Several bacterial genera (e.g., Halomonas, Marinomonas) were strongly associated with impaired larval performance while the presence of genera in larvae including Vibrio was closely associated with overfeeding. Our research demonstrated that metabarcoding can be effectively used to identify microbiota features associated with larval fitness.https://www.frontiersin.org/articles/10.3389/fmicb.2021.706214/fulloyster hatcherylarvaeCrassostrea gigasmicroalgaefitnessmicrobiome |
| spellingShingle | Julien Vignier Olivier Laroche Anne Rolton Pandora Wadsworth Karthiga Kumanan Branwen Trochel Xavier Pochon Xavier Pochon Nick King Dietary Exposure of Pacific Oyster (Crassostrea gigas) Larvae to Compromised Microalgae Results in Impaired Fitness and Microbiome Shift Frontiers in Microbiology oyster hatchery larvae Crassostrea gigas microalgae fitness microbiome |
| title | Dietary Exposure of Pacific Oyster (Crassostrea gigas) Larvae to Compromised Microalgae Results in Impaired Fitness and Microbiome Shift |
| title_full | Dietary Exposure of Pacific Oyster (Crassostrea gigas) Larvae to Compromised Microalgae Results in Impaired Fitness and Microbiome Shift |
| title_fullStr | Dietary Exposure of Pacific Oyster (Crassostrea gigas) Larvae to Compromised Microalgae Results in Impaired Fitness and Microbiome Shift |
| title_full_unstemmed | Dietary Exposure of Pacific Oyster (Crassostrea gigas) Larvae to Compromised Microalgae Results in Impaired Fitness and Microbiome Shift |
| title_short | Dietary Exposure of Pacific Oyster (Crassostrea gigas) Larvae to Compromised Microalgae Results in Impaired Fitness and Microbiome Shift |
| title_sort | dietary exposure of pacific oyster crassostrea gigas larvae to compromised microalgae results in impaired fitness and microbiome shift |
| topic | oyster hatchery larvae Crassostrea gigas microalgae fitness microbiome |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.706214/full |
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