CRISPR-Based Editing Techniques for Genetic Manipulation of Primary T Cells

While clustered regularly interspaced short palindromic repeats (CRISPR)-based genome editing techniques have been widely adapted for use in immortalised immune cells, efficient manipulation of primary T cells has proved to be more challenging. Nonetheless, the rapid expansion of the CRISPR toolbox...

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Bibliographic Details
Main Authors: Mateusz Kotowski, Sumana Sharma
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:Methods and Protocols
Subjects:
Online Access:https://www.mdpi.com/2409-9279/3/4/79