CRISPR-Based Editing Techniques for Genetic Manipulation of Primary T Cells
While clustered regularly interspaced short palindromic repeats (CRISPR)-based genome editing techniques have been widely adapted for use in immortalised immune cells, efficient manipulation of primary T cells has proved to be more challenging. Nonetheless, the rapid expansion of the CRISPR toolbox...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Methods and Protocols |
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| Online Access: | https://www.mdpi.com/2409-9279/3/4/79 |
| _version_ | 1797547586474737664 |
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| author | Mateusz Kotowski Sumana Sharma |
| author_facet | Mateusz Kotowski Sumana Sharma |
| author_sort | Mateusz Kotowski |
| collection | DOAJ |
| description | While clustered regularly interspaced short palindromic repeats (CRISPR)-based genome editing techniques have been widely adapted for use in immortalised immune cells, efficient manipulation of primary T cells has proved to be more challenging. Nonetheless, the rapid expansion of the CRISPR toolbox accompanied by the development of techniques for delivery of CRISPR components into primary T cells now affords the possibility to genetically manipulate primary T cells both with precision and at scale. Here, we review the key features of the techniques for primary T cell editing and discuss how the new generation of CRISPR-based tools may advance genetic engineering of these immune cells. This improved ability to genetically manipulate primary T cells will further enhance our fundamental understanding of cellular signalling and transcriptional networks in T cells and more importantly has the potential to revolutionise T cell-based therapies. |
| first_indexed | 2024-03-10T14:47:09Z |
| format | Article |
| id | doaj.art-cc250d606aa54df387c1ace39f61d36c |
| institution | Directory Open Access Journal |
| issn | 2409-9279 |
| language | English |
| last_indexed | 2024-03-10T14:47:09Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Methods and Protocols |
| spelling | doaj.art-cc250d606aa54df387c1ace39f61d36c2023-11-20T21:21:04ZengMDPI AGMethods and Protocols2409-92792020-11-01347910.3390/mps3040079CRISPR-Based Editing Techniques for Genetic Manipulation of Primary T CellsMateusz Kotowski0Sumana Sharma1MRC Human Immunology Unit, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DS, UKMRC Human Immunology Unit, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DS, UKWhile clustered regularly interspaced short palindromic repeats (CRISPR)-based genome editing techniques have been widely adapted for use in immortalised immune cells, efficient manipulation of primary T cells has proved to be more challenging. Nonetheless, the rapid expansion of the CRISPR toolbox accompanied by the development of techniques for delivery of CRISPR components into primary T cells now affords the possibility to genetically manipulate primary T cells both with precision and at scale. Here, we review the key features of the techniques for primary T cell editing and discuss how the new generation of CRISPR-based tools may advance genetic engineering of these immune cells. This improved ability to genetically manipulate primary T cells will further enhance our fundamental understanding of cellular signalling and transcriptional networks in T cells and more importantly has the potential to revolutionise T cell-based therapies.https://www.mdpi.com/2409-9279/3/4/79primary T cellsCRISPR/Cas9genome-editingCAR-T cells |
| spellingShingle | Mateusz Kotowski Sumana Sharma CRISPR-Based Editing Techniques for Genetic Manipulation of Primary T Cells Methods and Protocols primary T cells CRISPR/Cas9 genome-editing CAR-T cells |
| title | CRISPR-Based Editing Techniques for Genetic Manipulation of Primary T Cells |
| title_full | CRISPR-Based Editing Techniques for Genetic Manipulation of Primary T Cells |
| title_fullStr | CRISPR-Based Editing Techniques for Genetic Manipulation of Primary T Cells |
| title_full_unstemmed | CRISPR-Based Editing Techniques for Genetic Manipulation of Primary T Cells |
| title_short | CRISPR-Based Editing Techniques for Genetic Manipulation of Primary T Cells |
| title_sort | crispr based editing techniques for genetic manipulation of primary t cells |
| topic | primary T cells CRISPR/Cas9 genome-editing CAR-T cells |
| url | https://www.mdpi.com/2409-9279/3/4/79 |
| work_keys_str_mv | AT mateuszkotowski crisprbasededitingtechniquesforgeneticmanipulationofprimarytcells AT sumanasharma crisprbasededitingtechniquesforgeneticmanipulationofprimarytcells |