<i>Cupriavidus necator</i>-Produced Polyhydroxybutyrate/Eudragit FS Hybrid Nanoparticles Mitigates Ulcerative Colitis via Colon-Targeted Delivery of Cyclosporine A
Polyhydroxybutyrate (PHB) has emerged as a novel material for replacing various plastics used in the medical field. However, its application as a drug-delivery carrier for colitis-targeted delivery has not been explored. In this study, we used biosynthesized PHB combined with Eudragit FS (EFS) and c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/12/2811 |
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| author | Juho Lee Aruzhan Saparbayeva Shwe Phyu Hlaing Dongmin Kwak Hyunwoo Kim Jihyun Kim Eun Hee Lee Jin-Wook Yoo |
| author_facet | Juho Lee Aruzhan Saparbayeva Shwe Phyu Hlaing Dongmin Kwak Hyunwoo Kim Jihyun Kim Eun Hee Lee Jin-Wook Yoo |
| author_sort | Juho Lee |
| collection | DOAJ |
| description | Polyhydroxybutyrate (PHB) has emerged as a novel material for replacing various plastics used in the medical field. However, its application as a drug-delivery carrier for colitis-targeted delivery has not been explored. In this study, we used biosynthesized PHB combined with Eudragit FS (EFS) and cyclosporine A (CSA) to develop pH-responsive controlled CSA-releasing nanoparticles (CSA-PENPs) for colitis-targeted drug delivery and demonstrated its enhanced therapeutic efficacy in a dextran sulfate sodium (DSS)-induced murine colitis model. PHB was successfully biosynthesized in the bacterium <i>Cupriavidus necator</i>, as demonstrated by <sup>1</sup>H-NMR and FT-IR analyses. CSA-PENPs were fabricated via the oil-in-water emulsion solvent evaporation method. Owing to the potent pH-responsive and sustained drug release properties provided by PHB and EFS, CSA-PENPs could deliver a sufficient amount of CSA to inflamed tissues in the distal colon; in contrast, CSA-loaded EFS nanoparticles displayed premature burst release before reaching the target site. Due to enhanced CSA delivery to colitis tissues, CSA-PENPs exhibited potent anti-inflammatory effects in the DSS-induced murine colitis model. Overall, CSA-PENPs could be a promising drug-delivery system for treating ulcerative colitis. |
| first_indexed | 2024-03-09T15:58:44Z |
| format | Article |
| id | doaj.art-cc2915805ab0466bbc51c00ff5c96d34 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T15:58:44Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-cc2915805ab0466bbc51c00ff5c96d342023-11-24T17:22:27ZengMDPI AGPharmaceutics1999-49232022-12-011412281110.3390/pharmaceutics14122811<i>Cupriavidus necator</i>-Produced Polyhydroxybutyrate/Eudragit FS Hybrid Nanoparticles Mitigates Ulcerative Colitis via Colon-Targeted Delivery of Cyclosporine AJuho Lee0Aruzhan Saparbayeva1Shwe Phyu Hlaing2Dongmin Kwak3Hyunwoo Kim4Jihyun Kim5Eun Hee Lee6Jin-Wook Yoo7College of Pharmacy, Pusan National University, Busan 46241, Republic of KoreaCollege of Pharmacy, Pusan National University, Busan 46241, Republic of KoreaCollege of Pharmacy, Pusan National University, Busan 46241, Republic of KoreaCollege of Pharmacy, Pusan National University, Busan 46241, Republic of KoreaCollege of Pharmacy, Pusan National University, Busan 46241, Republic of KoreaCollege of Pharmacy, Pusan National University, Busan 46241, Republic of KoreaCollege of Pharmacy, Korea University, Sejong 30019, Republic of KoreaCollege of Pharmacy, Pusan National University, Busan 46241, Republic of KoreaPolyhydroxybutyrate (PHB) has emerged as a novel material for replacing various plastics used in the medical field. However, its application as a drug-delivery carrier for colitis-targeted delivery has not been explored. In this study, we used biosynthesized PHB combined with Eudragit FS (EFS) and cyclosporine A (CSA) to develop pH-responsive controlled CSA-releasing nanoparticles (CSA-PENPs) for colitis-targeted drug delivery and demonstrated its enhanced therapeutic efficacy in a dextran sulfate sodium (DSS)-induced murine colitis model. PHB was successfully biosynthesized in the bacterium <i>Cupriavidus necator</i>, as demonstrated by <sup>1</sup>H-NMR and FT-IR analyses. CSA-PENPs were fabricated via the oil-in-water emulsion solvent evaporation method. Owing to the potent pH-responsive and sustained drug release properties provided by PHB and EFS, CSA-PENPs could deliver a sufficient amount of CSA to inflamed tissues in the distal colon; in contrast, CSA-loaded EFS nanoparticles displayed premature burst release before reaching the target site. Due to enhanced CSA delivery to colitis tissues, CSA-PENPs exhibited potent anti-inflammatory effects in the DSS-induced murine colitis model. Overall, CSA-PENPs could be a promising drug-delivery system for treating ulcerative colitis.https://www.mdpi.com/1999-4923/14/12/2811polyhydroxybutyratepH-responsiveulcerative colitiscolitis-targeted drug deliverysustained releasePHB biosynthesis |
| spellingShingle | Juho Lee Aruzhan Saparbayeva Shwe Phyu Hlaing Dongmin Kwak Hyunwoo Kim Jihyun Kim Eun Hee Lee Jin-Wook Yoo <i>Cupriavidus necator</i>-Produced Polyhydroxybutyrate/Eudragit FS Hybrid Nanoparticles Mitigates Ulcerative Colitis via Colon-Targeted Delivery of Cyclosporine A Pharmaceutics polyhydroxybutyrate pH-responsive ulcerative colitis colitis-targeted drug delivery sustained release PHB biosynthesis |
| title | <i>Cupriavidus necator</i>-Produced Polyhydroxybutyrate/Eudragit FS Hybrid Nanoparticles Mitigates Ulcerative Colitis via Colon-Targeted Delivery of Cyclosporine A |
| title_full | <i>Cupriavidus necator</i>-Produced Polyhydroxybutyrate/Eudragit FS Hybrid Nanoparticles Mitigates Ulcerative Colitis via Colon-Targeted Delivery of Cyclosporine A |
| title_fullStr | <i>Cupriavidus necator</i>-Produced Polyhydroxybutyrate/Eudragit FS Hybrid Nanoparticles Mitigates Ulcerative Colitis via Colon-Targeted Delivery of Cyclosporine A |
| title_full_unstemmed | <i>Cupriavidus necator</i>-Produced Polyhydroxybutyrate/Eudragit FS Hybrid Nanoparticles Mitigates Ulcerative Colitis via Colon-Targeted Delivery of Cyclosporine A |
| title_short | <i>Cupriavidus necator</i>-Produced Polyhydroxybutyrate/Eudragit FS Hybrid Nanoparticles Mitigates Ulcerative Colitis via Colon-Targeted Delivery of Cyclosporine A |
| title_sort | i cupriavidus necator i produced polyhydroxybutyrate eudragit fs hybrid nanoparticles mitigates ulcerative colitis via colon targeted delivery of cyclosporine a |
| topic | polyhydroxybutyrate pH-responsive ulcerative colitis colitis-targeted drug delivery sustained release PHB biosynthesis |
| url | https://www.mdpi.com/1999-4923/14/12/2811 |
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