RhoGDIβ-induced hypertrophic growth in H9c2 cells is negatively regulated by ZAK
<p>Abstract</p> <p>We found that overexpression of RhoGDIβ, a Rho GDP dissociation inhibitor, induced hypertrophic growth and suppressed cell cycle progression in a cultured cardiomyoblast cell line. Knockdown of RhoGDIβ expression by RNA interference blocked hypertrophic growth. W...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-01-01
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| Series: | Journal of Biomedical Science |
| Online Access: | http://www.jbiomedsci.com/content/16/1/11 |
| _version_ | 1819123114278125568 |
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| author | Huang Chih-Yang Yang Li-Chiu Liu Kuan-Yu Liao Pao-Hsin Chou Janet Chou Ming-Yung Lin Wei-Wen Yang Jaw-Ji |
| author_facet | Huang Chih-Yang Yang Li-Chiu Liu Kuan-Yu Liao Pao-Hsin Chou Janet Chou Ming-Yung Lin Wei-Wen Yang Jaw-Ji |
| author_sort | Huang Chih-Yang |
| collection | DOAJ |
| description | <p>Abstract</p> <p>We found that overexpression of RhoGDIβ, a Rho GDP dissociation inhibitor, induced hypertrophic growth and suppressed cell cycle progression in a cultured cardiomyoblast cell line. Knockdown of RhoGDIβ expression by RNA interference blocked hypertrophic growth. We further demonstrated that RhoGDIβ physically interacts with ZAK and is phosphorylated by ZAK <it>in vitro</it>, and this phosphorylation negatively regulates RhoGDIβ functions. Moreover, the ZAK-RhoGDIβ interaction may maintain ZAK in an inactive hypophosphorylated form. These two proteins could negatively regulate one another such that ZAK suppresses RhoGDIβ functions through phosphorylation and RhoGDIβ counteracts the effects of ZAK by physical interaction. Knockdown of ZAK expression in ZAK- and RhoGDIβ-expressing cells by ZAK-specific RNA interference restored the full functions of RhoGDIβ.</p> |
| first_indexed | 2024-12-22T07:03:12Z |
| format | Article |
| id | doaj.art-cc5a581937db4ce7a43be56dcef06864 |
| institution | Directory Open Access Journal |
| issn | 1021-7770 1423-0127 |
| language | English |
| last_indexed | 2024-12-22T07:03:12Z |
| publishDate | 2009-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biomedical Science |
| spelling | doaj.art-cc5a581937db4ce7a43be56dcef068642022-12-21T18:34:45ZengBMCJournal of Biomedical Science1021-77701423-01272009-01-011611110.1186/1423-0127-16-11RhoGDIβ-induced hypertrophic growth in H9c2 cells is negatively regulated by ZAKHuang Chih-YangYang Li-ChiuLiu Kuan-YuLiao Pao-HsinChou JanetChou Ming-YungLin Wei-WenYang Jaw-Ji<p>Abstract</p> <p>We found that overexpression of RhoGDIβ, a Rho GDP dissociation inhibitor, induced hypertrophic growth and suppressed cell cycle progression in a cultured cardiomyoblast cell line. Knockdown of RhoGDIβ expression by RNA interference blocked hypertrophic growth. We further demonstrated that RhoGDIβ physically interacts with ZAK and is phosphorylated by ZAK <it>in vitro</it>, and this phosphorylation negatively regulates RhoGDIβ functions. Moreover, the ZAK-RhoGDIβ interaction may maintain ZAK in an inactive hypophosphorylated form. These two proteins could negatively regulate one another such that ZAK suppresses RhoGDIβ functions through phosphorylation and RhoGDIβ counteracts the effects of ZAK by physical interaction. Knockdown of ZAK expression in ZAK- and RhoGDIβ-expressing cells by ZAK-specific RNA interference restored the full functions of RhoGDIβ.</p>http://www.jbiomedsci.com/content/16/1/11 |
| spellingShingle | Huang Chih-Yang Yang Li-Chiu Liu Kuan-Yu Liao Pao-Hsin Chou Janet Chou Ming-Yung Lin Wei-Wen Yang Jaw-Ji RhoGDIβ-induced hypertrophic growth in H9c2 cells is negatively regulated by ZAK Journal of Biomedical Science |
| title | RhoGDIβ-induced hypertrophic growth in H9c2 cells is negatively regulated by ZAK |
| title_full | RhoGDIβ-induced hypertrophic growth in H9c2 cells is negatively regulated by ZAK |
| title_fullStr | RhoGDIβ-induced hypertrophic growth in H9c2 cells is negatively regulated by ZAK |
| title_full_unstemmed | RhoGDIβ-induced hypertrophic growth in H9c2 cells is negatively regulated by ZAK |
| title_short | RhoGDIβ-induced hypertrophic growth in H9c2 cells is negatively regulated by ZAK |
| title_sort | rhogdiβ induced hypertrophic growth in h9c2 cells is negatively regulated by zak |
| url | http://www.jbiomedsci.com/content/16/1/11 |
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