Dose Optimization of Calcusol™ and Calcium Oxalate Monohydrate (COM) on Primary Renal Epithelial Cells Cultures of Mice ( Mus musculus )
Kidney stones are one of the urologic diseases that have plagued mankind for centuries. The main constituents of stones in the kidney are calcium oxalate monohydrate (COM) crystals. Nowadays, there are varieties of drugs and treatments that can be made to minimize the grievances due t...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Brawijaya
2014-05-01
|
| Series: | Journal of Tropical Life Science |
| Subjects: | |
| Online Access: | http://www.jtrolis.ub.ac.id/index.php/jtrolis/article/view/162/199 |
| _version_ | 1827613193679142912 |
|---|---|
| author | Ahmad Soni Sasmito Djati Sri Widyarti |
| author_facet | Ahmad Soni Sasmito Djati Sri Widyarti |
| author_sort | Ahmad Soni |
| collection | DOAJ |
| description | Kidney stones are one of the urologic diseases that have plagued mankind for centuries. The main constituents of stones in the kidney are calcium oxalate monohydrate (COM) crystals. Nowadays, there are varieties of drugs and treatments that can be made to minimize the grievances due to kidney stone disease. The treatment can be done either by using chemicals or traditional medicine. Calcusol™ is one of the popular herbal products that have been used by Indonesian people in curing the kidney stone disease. The main constituent that was contained in Calcusol™ is an extract of the tempuyung leaves (Sonchus arvensis L.), which is expected could cure the kidney stone disease. This study used primary cultured renal epithelial cells of mice to determine the optimal dose of Calcusol™ and the optimal dose of COM. The primary Kidney epithelial cell were treated with Calcusol™ and COM at various doses. The analysis of the cell death either by necrosis or apoptosis pathways was analyzed by flow cytometric analysis. The results that were obtained is the percentage of cell death that is then analyzed by using a complete randomized design (CRD) One Way Anova. Based on the results that were obtained, it is known that the optimal dose of Calcusol™ in vitro were ranging from 75 ppm to 100 ppm, whereas the optimal dose of COM suggested for 500 ppm. |
| first_indexed | 2024-03-09T08:35:18Z |
| format | Article |
| id | doaj.art-cc63a688d5b444e7a6bf60c4183fcd1c |
| institution | Directory Open Access Journal |
| issn | 2087-5517 |
| language | English |
| last_indexed | 2024-03-09T08:35:18Z |
| publishDate | 2014-05-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Tropical Life Science |
| spelling | doaj.art-cc63a688d5b444e7a6bf60c4183fcd1c2023-12-02T18:23:05ZengUniversity of BrawijayaJournal of Tropical Life Science2087-55172014-05-0142101105Dose Optimization of Calcusol™ and Calcium Oxalate Monohydrate (COM) on Primary Renal Epithelial Cells Cultures of Mice ( Mus musculus )Ahmad Soni0 Sasmito Djati1 Sri Widyarti2Department of Biology, Faculty of Mathematics and Natural Sciences Universitas Brawijaya, MalangDepartment of Biology, Faculty of Mathematics and Natural Sciences Universitas Brawijaya, MalangDepartment of Biology, Faculty of Mathematics and Natural Sciences Universitas Brawijaya, MalangKidney stones are one of the urologic diseases that have plagued mankind for centuries. The main constituents of stones in the kidney are calcium oxalate monohydrate (COM) crystals. Nowadays, there are varieties of drugs and treatments that can be made to minimize the grievances due to kidney stone disease. The treatment can be done either by using chemicals or traditional medicine. Calcusol™ is one of the popular herbal products that have been used by Indonesian people in curing the kidney stone disease. The main constituent that was contained in Calcusol™ is an extract of the tempuyung leaves (Sonchus arvensis L.), which is expected could cure the kidney stone disease. This study used primary cultured renal epithelial cells of mice to determine the optimal dose of Calcusol™ and the optimal dose of COM. The primary Kidney epithelial cell were treated with Calcusol™ and COM at various doses. The analysis of the cell death either by necrosis or apoptosis pathways was analyzed by flow cytometric analysis. The results that were obtained is the percentage of cell death that is then analyzed by using a complete randomized design (CRD) One Way Anova. Based on the results that were obtained, it is known that the optimal dose of Calcusol™ in vitro were ranging from 75 ppm to 100 ppm, whereas the optimal dose of COM suggested for 500 ppm.http://www.jtrolis.ub.ac.id/index.php/jtrolis/article/view/162/199Calcusol™COMkidney stonestempuyung |
| spellingShingle | Ahmad Soni Sasmito Djati Sri Widyarti Dose Optimization of Calcusol™ and Calcium Oxalate Monohydrate (COM) on Primary Renal Epithelial Cells Cultures of Mice ( Mus musculus ) Journal of Tropical Life Science Calcusol™ COM kidney stones tempuyung |
| title | Dose Optimization of Calcusol™ and Calcium Oxalate Monohydrate (COM) on Primary Renal Epithelial Cells Cultures of Mice ( Mus musculus ) |
| title_full | Dose Optimization of Calcusol™ and Calcium Oxalate Monohydrate (COM) on Primary Renal Epithelial Cells Cultures of Mice ( Mus musculus ) |
| title_fullStr | Dose Optimization of Calcusol™ and Calcium Oxalate Monohydrate (COM) on Primary Renal Epithelial Cells Cultures of Mice ( Mus musculus ) |
| title_full_unstemmed | Dose Optimization of Calcusol™ and Calcium Oxalate Monohydrate (COM) on Primary Renal Epithelial Cells Cultures of Mice ( Mus musculus ) |
| title_short | Dose Optimization of Calcusol™ and Calcium Oxalate Monohydrate (COM) on Primary Renal Epithelial Cells Cultures of Mice ( Mus musculus ) |
| title_sort | dose optimization of calcusol™ and calcium oxalate monohydrate com on primary renal epithelial cells cultures of mice mus musculus |
| topic | Calcusol™ COM kidney stones tempuyung |
| url | http://www.jtrolis.ub.ac.id/index.php/jtrolis/article/view/162/199 |
| work_keys_str_mv | AT ahmadsoni doseoptimizationofcalcusolandcalciumoxalatemonohydratecomonprimaryrenalepithelialcellsculturesofmicemusmusculus AT sasmitodjati doseoptimizationofcalcusolandcalciumoxalatemonohydratecomonprimaryrenalepithelialcellsculturesofmicemusmusculus AT sriwidyarti doseoptimizationofcalcusolandcalciumoxalatemonohydratecomonprimaryrenalepithelialcellsculturesofmicemusmusculus |