Protective Effect of <i>Prunus mume</i> Fermented with Mixed Lactic Acid Bacteria in Dextran Sodium Sulfate-Induced Colitis
The fruit of <i>Prunus mume</i> (PM) is widely cultivated in East Asia, and it has been used as a folk medication for gastrointestinal disorders, e.g., diarrhea, stomach ache and ulceration. In this study, the pectinase-treated PM juice (PJ) was fermented with <i>Lactobacillus</...
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MDPI AG
2020-12-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/1/58 |
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| author | Jeong-Ho Kim Yeong-Seon Won Hyun-Dong Cho Seong-Min Hong Kwang-Deog Moon Kwon-Il Seo |
| author_facet | Jeong-Ho Kim Yeong-Seon Won Hyun-Dong Cho Seong-Min Hong Kwang-Deog Moon Kwon-Il Seo |
| author_sort | Jeong-Ho Kim |
| collection | DOAJ |
| description | The fruit of <i>Prunus mume</i> (PM) is widely cultivated in East Asia, and it has been used as a folk medication for gastrointestinal disorders, e.g., diarrhea, stomach ache and ulceration. In this study, the pectinase-treated PM juice (PJ) was fermented with <i>Lactobacillus</i> strains containing fundamental organic acids and free amino acids. The PJ fermented with <i>Lactobacillus plantarum</i> and <i>L. casei</i> (FP) was investigated for its protective effect in dextran sodium sulfate (DSS)-induced colitis mice model. The administration of FP reduced lipid peroxidation and histopathological colitis symptoms, e.g., shortening of the colon length, depletion of mucin, epithelial injury and ulceration, in colonic tissues. The FP-supplemented group showed the alleviation of pro-inflammatory cytokines. Compared with the DSS control group, the supplementation of FP significantly reduced the levels of serum interferon-γ (IFN-γ), interleukin (IL)-1β, IL-6, IL-12 and IL-17 as well as colonic tumor necrosis factor-α, IFN-γ, IL-12 and IL-17. Furthermore, the DSS-induced TUNEL-positive area was significantly reduced by the FP supplementation. These results show that the supplementation of FP fermented with mixed lactic acid bacteria, <i>L. plantarum</i> and <i>L. casei</i>, elucidated the protective effect in DSS-induced colitis mice. Hence, this study suggests that FP can be utilized as a natural therapeutic agent for colitis and intestinal inflammation. |
| first_indexed | 2024-03-10T13:42:03Z |
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| last_indexed | 2024-03-10T13:42:03Z |
| publishDate | 2020-12-01 |
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| series | Foods |
| spelling | doaj.art-cc6539ae9ada4c2bbe3477e4299d217f2023-11-21T02:57:45ZengMDPI AGFoods2304-81582020-12-011015810.3390/foods10010058Protective Effect of <i>Prunus mume</i> Fermented with Mixed Lactic Acid Bacteria in Dextran Sodium Sulfate-Induced ColitisJeong-Ho Kim0Yeong-Seon Won1Hyun-Dong Cho2Seong-Min Hong3Kwang-Deog Moon4Kwon-Il Seo5Department of Food Science and Technology, Kyungpook National University, Daegu 41566, KoreaDepartment of Food Biotechnology, Dong-A University, Busan 49315, KoreaDepartment of Pharmacology & Regenerative Medicine, University of Illinois, Chicago, IL 60612, USACollege of Pharmacy and Gachon Institute of Pharmaceutical Science, Gachon University, Incheon 21936, KoreaDepartment of Food Science and Technology, Kyungpook National University, Daegu 41566, KoreaDepartment of Food Biotechnology, Dong-A University, Busan 49315, KoreaThe fruit of <i>Prunus mume</i> (PM) is widely cultivated in East Asia, and it has been used as a folk medication for gastrointestinal disorders, e.g., diarrhea, stomach ache and ulceration. In this study, the pectinase-treated PM juice (PJ) was fermented with <i>Lactobacillus</i> strains containing fundamental organic acids and free amino acids. The PJ fermented with <i>Lactobacillus plantarum</i> and <i>L. casei</i> (FP) was investigated for its protective effect in dextran sodium sulfate (DSS)-induced colitis mice model. The administration of FP reduced lipid peroxidation and histopathological colitis symptoms, e.g., shortening of the colon length, depletion of mucin, epithelial injury and ulceration, in colonic tissues. The FP-supplemented group showed the alleviation of pro-inflammatory cytokines. Compared with the DSS control group, the supplementation of FP significantly reduced the levels of serum interferon-γ (IFN-γ), interleukin (IL)-1β, IL-6, IL-12 and IL-17 as well as colonic tumor necrosis factor-α, IFN-γ, IL-12 and IL-17. Furthermore, the DSS-induced TUNEL-positive area was significantly reduced by the FP supplementation. These results show that the supplementation of FP fermented with mixed lactic acid bacteria, <i>L. plantarum</i> and <i>L. casei</i>, elucidated the protective effect in DSS-induced colitis mice. Hence, this study suggests that FP can be utilized as a natural therapeutic agent for colitis and intestinal inflammation.https://www.mdpi.com/2304-8158/10/1/58colitisfermentationlactic acid bacteriapro-inflammatory cytokine<i>Prunus mume</i> |
| spellingShingle | Jeong-Ho Kim Yeong-Seon Won Hyun-Dong Cho Seong-Min Hong Kwang-Deog Moon Kwon-Il Seo Protective Effect of <i>Prunus mume</i> Fermented with Mixed Lactic Acid Bacteria in Dextran Sodium Sulfate-Induced Colitis Foods colitis fermentation lactic acid bacteria pro-inflammatory cytokine <i>Prunus mume</i> |
| title | Protective Effect of <i>Prunus mume</i> Fermented with Mixed Lactic Acid Bacteria in Dextran Sodium Sulfate-Induced Colitis |
| title_full | Protective Effect of <i>Prunus mume</i> Fermented with Mixed Lactic Acid Bacteria in Dextran Sodium Sulfate-Induced Colitis |
| title_fullStr | Protective Effect of <i>Prunus mume</i> Fermented with Mixed Lactic Acid Bacteria in Dextran Sodium Sulfate-Induced Colitis |
| title_full_unstemmed | Protective Effect of <i>Prunus mume</i> Fermented with Mixed Lactic Acid Bacteria in Dextran Sodium Sulfate-Induced Colitis |
| title_short | Protective Effect of <i>Prunus mume</i> Fermented with Mixed Lactic Acid Bacteria in Dextran Sodium Sulfate-Induced Colitis |
| title_sort | protective effect of i prunus mume i fermented with mixed lactic acid bacteria in dextran sodium sulfate induced colitis |
| topic | colitis fermentation lactic acid bacteria pro-inflammatory cytokine <i>Prunus mume</i> |
| url | https://www.mdpi.com/2304-8158/10/1/58 |
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