Overload Induced Heat Shock Proteins (HSPs), MAPK and miRNA (miR-1 and miR133a) Response in Insulin-Resistant Skeletal Muscle
Background: Insulin resistance (IR) may decrease muscle adaptability. Heat shock proteins (HSPs), mitogen-activated protein kinases (MAPKs), and miRNA are thought to play a role in muscle hypertrophy but it is unclear if IR may affect their regulation. Methods: Soleus muscles of lean Zucker (LZ) and...
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2013-02-01
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| Series: | Cellular Physiology and Biochemistry |
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| Online Access: | http://www.karger.com/Article/FullText/343363 |
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| author | Anjaiah Katta Srinivasarao Thulluri Nandini D.P.K. Manne Hari S. Addagarla Ravikumar Arvapalli Siva K. Nalabotu Muralikrishna Gadde Kevin M. Rice Eric R. Blough |
| author_facet | Anjaiah Katta Srinivasarao Thulluri Nandini D.P.K. Manne Hari S. Addagarla Ravikumar Arvapalli Siva K. Nalabotu Muralikrishna Gadde Kevin M. Rice Eric R. Blough |
| author_sort | Anjaiah Katta |
| collection | DOAJ |
| description | Background: Insulin resistance (IR) may decrease muscle adaptability. Heat shock proteins (HSPs), mitogen-activated protein kinases (MAPKs), and miRNA are thought to play a role in muscle hypertrophy but it is unclear if IR may affect their regulation. Methods: Soleus muscles of lean Zucker (LZ) and insulin resistant obese Zucker (OZ) rats were overloaded for 7 or 21 days and subjected to immunoblotting and RT-PCR. Results: IR was associated with decreased muscle hypertrophy. Overload increased HSP27 phosphorylation in both the LZ and OZ rats at day 7 but only in the LZ at day 21. IR was associated with diminished overload induced MAPK phosphorylation and decreased expression of miR-1 and miR133. Overload decreased mir-1 levels in both the LZ and OZ but to a greater extent in the LZ animals. Conclusion: These results suggest that alterations in the regulation of HSPs, MAPKs and miRNA may be associated with the diminished hypertrophy of IR muscle. |
| first_indexed | 2024-12-10T22:36:28Z |
| format | Article |
| id | doaj.art-cc68f51b7cdc407a933865cce917db46 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-10T22:36:28Z |
| publishDate | 2013-02-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-cc68f51b7cdc407a933865cce917db462022-12-22T01:30:52ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782013-02-01312-321922910.1159/000343363343363Overload Induced Heat Shock Proteins (HSPs), MAPK and miRNA (miR-1 and miR133a) Response in Insulin-Resistant Skeletal MuscleAnjaiah KattaSrinivasarao ThulluriNandini D.P.K. ManneHari S. AddagarlaRavikumar ArvapalliSiva K. NalabotuMuralikrishna GaddeKevin M. RiceEric R. BloughBackground: Insulin resistance (IR) may decrease muscle adaptability. Heat shock proteins (HSPs), mitogen-activated protein kinases (MAPKs), and miRNA are thought to play a role in muscle hypertrophy but it is unclear if IR may affect their regulation. Methods: Soleus muscles of lean Zucker (LZ) and insulin resistant obese Zucker (OZ) rats were overloaded for 7 or 21 days and subjected to immunoblotting and RT-PCR. Results: IR was associated with decreased muscle hypertrophy. Overload increased HSP27 phosphorylation in both the LZ and OZ rats at day 7 but only in the LZ at day 21. IR was associated with diminished overload induced MAPK phosphorylation and decreased expression of miR-1 and miR133. Overload decreased mir-1 levels in both the LZ and OZ but to a greater extent in the LZ animals. Conclusion: These results suggest that alterations in the regulation of HSPs, MAPKs and miRNA may be associated with the diminished hypertrophy of IR muscle.http://www.karger.com/Article/FullText/343363Heat shock proteinsMitogen-activated protein kinasesmiRNAInsulin resistanceMuscle overload |
| spellingShingle | Anjaiah Katta Srinivasarao Thulluri Nandini D.P.K. Manne Hari S. Addagarla Ravikumar Arvapalli Siva K. Nalabotu Muralikrishna Gadde Kevin M. Rice Eric R. Blough Overload Induced Heat Shock Proteins (HSPs), MAPK and miRNA (miR-1 and miR133a) Response in Insulin-Resistant Skeletal Muscle Cellular Physiology and Biochemistry Heat shock proteins Mitogen-activated protein kinases miRNA Insulin resistance Muscle overload |
| title | Overload Induced Heat Shock Proteins (HSPs), MAPK and miRNA (miR-1 and miR133a) Response in Insulin-Resistant Skeletal Muscle |
| title_full | Overload Induced Heat Shock Proteins (HSPs), MAPK and miRNA (miR-1 and miR133a) Response in Insulin-Resistant Skeletal Muscle |
| title_fullStr | Overload Induced Heat Shock Proteins (HSPs), MAPK and miRNA (miR-1 and miR133a) Response in Insulin-Resistant Skeletal Muscle |
| title_full_unstemmed | Overload Induced Heat Shock Proteins (HSPs), MAPK and miRNA (miR-1 and miR133a) Response in Insulin-Resistant Skeletal Muscle |
| title_short | Overload Induced Heat Shock Proteins (HSPs), MAPK and miRNA (miR-1 and miR133a) Response in Insulin-Resistant Skeletal Muscle |
| title_sort | overload induced heat shock proteins hsps mapk and mirna mir 1 and mir133a response in insulin resistant skeletal muscle |
| topic | Heat shock proteins Mitogen-activated protein kinases miRNA Insulin resistance Muscle overload |
| url | http://www.karger.com/Article/FullText/343363 |
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