Development and laboratory evaluation of a competitive ELISA for serodiagnosis of Nipah and Hendra virus infection using recombinant Nipah glycoproteins and a monoclonal antibody
IntroductionNipah virus (NiV) and Hendra virus (HeV), of the genus Henipavirus, family Paramyxoviridae, are classified as Risk Group 4 (RG4) pathogens that cause respiratory disease in pigs and acute/febrile encephalitis in humans with high mortality.MethodsA competitive enzyme–linked immunosorbent...
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| Format: | Article |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1120367/full |
| _version_ | 1797937925867962368 |
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| author | Wenjun Zhu Bradley Pickering Bradley Pickering Bradley Pickering Greg Smith Mathieu Pinette Thang Truong Shawn Babiuk Shawn Babiuk Darwyn Kobasa Darwyn Kobasa Logan Banadyga Logan Banadyga Ming Yang |
| author_facet | Wenjun Zhu Bradley Pickering Bradley Pickering Bradley Pickering Greg Smith Mathieu Pinette Thang Truong Shawn Babiuk Shawn Babiuk Darwyn Kobasa Darwyn Kobasa Logan Banadyga Logan Banadyga Ming Yang |
| author_sort | Wenjun Zhu |
| collection | DOAJ |
| description | IntroductionNipah virus (NiV) and Hendra virus (HeV), of the genus Henipavirus, family Paramyxoviridae, are classified as Risk Group 4 (RG4) pathogens that cause respiratory disease in pigs and acute/febrile encephalitis in humans with high mortality.MethodsA competitive enzyme–linked immunosorbent assay (cELISA) using a monoclonal antibody (mAb) and recombinant NiV glycoprotein (G) was developed and laboratory evaluated using sera from experimental pigs, mini pigs and nonhuman primates. The test depends on competition between specific antibodies in positive sera and a virus–specific mAb for binding to NiV–G.ResultsBased on 1,199 negative and 71 NiV positive serum test results, the cutoff value was determined as 35% inhibition. The diagnostic sensitivity and specificity of the NiV cELISA was 98.58 and 99.92%, respectively. When testing sera from animals experimentally infected with NiV Malaysia, the cELISA detected antibodies from 14 days post–infection (dpi) and remained positive until the end of the experiment (28 dpi). Comparisons using the Kappa coefficient showed strong agreement (100%) between the cELISA and a plaque reduction neutralization test (PRNT).DiscussionBecause our cELISA is simpler, faster, and gives comparable or better results than PRNT, it would be an adequate screening test for suspect NiV and HeV cases, and it would also be useful for epidemiological surveillance of Henipavirus infections in different animal species without changing reagents. |
| first_indexed | 2024-04-10T18:51:50Z |
| format | Article |
| id | doaj.art-cc6a73e8c80c4ebea86c5ee22946f571 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-10T18:51:50Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-cc6a73e8c80c4ebea86c5ee22946f5712023-02-01T06:52:40ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-02-011010.3389/fvets.2023.11203671120367Development and laboratory evaluation of a competitive ELISA for serodiagnosis of Nipah and Hendra virus infection using recombinant Nipah glycoproteins and a monoclonal antibodyWenjun Zhu0Bradley Pickering1Bradley Pickering2Bradley Pickering3Greg Smith4Mathieu Pinette5Thang Truong6Shawn Babiuk7Shawn Babiuk8Darwyn Kobasa9Darwyn Kobasa10Logan Banadyga11Logan Banadyga12Ming Yang13National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, CanadaNational Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, CanadaDepartment of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB, CanadaDepartment of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, United StatesNational Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, CanadaNational Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, CanadaNational Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, CanadaNational Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, CanadaDepartment of Immunology, University of Manitoba, Winnipeg, MB, CanadaDepartment of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB, CanadaNational Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, CanadaDepartment of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB, CanadaNational Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, CanadaNational Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, CanadaIntroductionNipah virus (NiV) and Hendra virus (HeV), of the genus Henipavirus, family Paramyxoviridae, are classified as Risk Group 4 (RG4) pathogens that cause respiratory disease in pigs and acute/febrile encephalitis in humans with high mortality.MethodsA competitive enzyme–linked immunosorbent assay (cELISA) using a monoclonal antibody (mAb) and recombinant NiV glycoprotein (G) was developed and laboratory evaluated using sera from experimental pigs, mini pigs and nonhuman primates. The test depends on competition between specific antibodies in positive sera and a virus–specific mAb for binding to NiV–G.ResultsBased on 1,199 negative and 71 NiV positive serum test results, the cutoff value was determined as 35% inhibition. The diagnostic sensitivity and specificity of the NiV cELISA was 98.58 and 99.92%, respectively. When testing sera from animals experimentally infected with NiV Malaysia, the cELISA detected antibodies from 14 days post–infection (dpi) and remained positive until the end of the experiment (28 dpi). Comparisons using the Kappa coefficient showed strong agreement (100%) between the cELISA and a plaque reduction neutralization test (PRNT).DiscussionBecause our cELISA is simpler, faster, and gives comparable or better results than PRNT, it would be an adequate screening test for suspect NiV and HeV cases, and it would also be useful for epidemiological surveillance of Henipavirus infections in different animal species without changing reagents.https://www.frontiersin.org/articles/10.3389/fvets.2023.1120367/fullNipah virusHendra virusHenipavirusrecombinant Nipah virus glycoproteinmonoclonal antibodycompetitive ELISA |
| spellingShingle | Wenjun Zhu Bradley Pickering Bradley Pickering Bradley Pickering Greg Smith Mathieu Pinette Thang Truong Shawn Babiuk Shawn Babiuk Darwyn Kobasa Darwyn Kobasa Logan Banadyga Logan Banadyga Ming Yang Development and laboratory evaluation of a competitive ELISA for serodiagnosis of Nipah and Hendra virus infection using recombinant Nipah glycoproteins and a monoclonal antibody Frontiers in Veterinary Science Nipah virus Hendra virus Henipavirus recombinant Nipah virus glycoprotein monoclonal antibody competitive ELISA |
| title | Development and laboratory evaluation of a competitive ELISA for serodiagnosis of Nipah and Hendra virus infection using recombinant Nipah glycoproteins and a monoclonal antibody |
| title_full | Development and laboratory evaluation of a competitive ELISA for serodiagnosis of Nipah and Hendra virus infection using recombinant Nipah glycoproteins and a monoclonal antibody |
| title_fullStr | Development and laboratory evaluation of a competitive ELISA for serodiagnosis of Nipah and Hendra virus infection using recombinant Nipah glycoproteins and a monoclonal antibody |
| title_full_unstemmed | Development and laboratory evaluation of a competitive ELISA for serodiagnosis of Nipah and Hendra virus infection using recombinant Nipah glycoproteins and a monoclonal antibody |
| title_short | Development and laboratory evaluation of a competitive ELISA for serodiagnosis of Nipah and Hendra virus infection using recombinant Nipah glycoproteins and a monoclonal antibody |
| title_sort | development and laboratory evaluation of a competitive elisa for serodiagnosis of nipah and hendra virus infection using recombinant nipah glycoproteins and a monoclonal antibody |
| topic | Nipah virus Hendra virus Henipavirus recombinant Nipah virus glycoprotein monoclonal antibody competitive ELISA |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1120367/full |
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