Creating a more robust 5-hydroxymethylfurfural oxidase by combining computational predictions with a novel effective library design

Creating a more robust 5-hydroxymethylfurfural oxidase by combining computational predictions with a novel effective library design

Abstract Background HMF oxidase (HMFO) from Methylovorus sp. is a recently characterized flavoprotein oxidase. HMFO is a remarkable enzyme as it is able to oxidize 5-hydroxymethylfurfural (HMF) into 2,5-furandicarboxylic acid (FDCA): a catalytic cascade of three oxidation steps. Because HMF can be f...

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Bibliographic Details
Main Authors: Caterina Martin, Amaury Ovalle Maqueo, Hein J. Wijma, Marco W. Fraaije
Format: Article
Language:English
Published: BMC 2018-03-01
Series:Biotechnology for Biofuels
Subjects:
Enzyme engineering
Carbohydrate
Poly(ethylene-furandicarboxylate) PEF
Computational library
Golden Gate gene shuffling
Online Access:http://link.springer.com/article/10.1186/s13068-018-1051-x

Internet

http://link.springer.com/article/10.1186/s13068-018-1051-x

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