Circular Whole-Transcriptome Amplification (cWTA) and mNGS Screening Enhanced by a Group Testing Algorithm (mEGA) Enable High-Throughput and Comprehensive Virus Identification

Circular Whole-Transcriptome Amplification (cWTA) and mNGS Screening Enhanced by a Group Testing Algorithm (mEGA) Enable High-Throughput and Comprehensive Virus Identification

ABSTRACT Metagenomic next-generation sequencing (mNGS) offers a hypothesis-free approach for pathogen detection, but its applicability in clinical diagnosis, in addition to other factors, remains limited due to complicated library construction. The present study describes a PCR-free isothermal workf...

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Bibliographic Details
Main Authors: Patrick Reteng, Linh Nguyen Thuy, Mizanur Rahman, Ana Maria Bispo de Filippis, Kyoko Hayashida, Tatsuki Sugi, Gabriel Gonzalez, William W. Hall, Lan Anh Nguyen Thi, Junya Yamagishi
Format: Article
Language:English
Published: American Society for Microbiology 2022-10-01
Series:mSphere
Subjects:
metagenomic
febrile illness
group testing algorithm
multiple displacement amplification
comprehensive pathogen detection
Online Access:https://journals.asm.org/doi/10.1128/msphere.00332-22

Internet

https://journals.asm.org/doi/10.1128/msphere.00332-22

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