Acetylation of p65Lys310 by p300 in macrophages mediates anti-inflammatory property of berberine
Nuclear factor (NF)-κB plays a pivotal role in the regulation of inflammatory response in macrophages. Berberine (BBR), which is an active constituent isolated from Coptis rhizome, possesses a prominent anti-inflammatory activity. Here we show that BBR changes the global acetylation landscape in LPS...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-06-01
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| Series: | Redox Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213231723001052 |
| _version_ | 1797830056142176256 |
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| author | Shuchen Zhang Pingyuan Xu Ziwei Zhu Lingyan Zhou Jiao Li Ruonan Zhou Yue Kan Yaru Li Xizhong Yu Juan Zhao Yu Jin Jing Yan Penghua Fang Wenbin Shang |
| author_facet | Shuchen Zhang Pingyuan Xu Ziwei Zhu Lingyan Zhou Jiao Li Ruonan Zhou Yue Kan Yaru Li Xizhong Yu Juan Zhao Yu Jin Jing Yan Penghua Fang Wenbin Shang |
| author_sort | Shuchen Zhang |
| collection | DOAJ |
| description | Nuclear factor (NF)-κB plays a pivotal role in the regulation of inflammatory response in macrophages. Berberine (BBR), which is an active constituent isolated from Coptis rhizome, possesses a prominent anti-inflammatory activity. Here we show that BBR changes the global acetylation landscape in LPS-induced protein acetylation of macrophages and reduces the acetylation of NF-κB subunit p65 at site Lys310(p65Lys310), leading to the inhibition of NF-κB translocation and transcriptional activity to suppress the expressions of inflammatory factors. BBR resists the inflammatory response in acute LPS-stimulated mice through downregulation of p65Lys310 acetylation in peritoneal macrophages. In obese mice, BBR alleviates the metabolic disorder and inflammation with the reduced acetylation of p65Lys310 in white adipose tissue. Furthermore, we demonstrate that BBR acts as a regulator of p65Lys310 by inhibiting the expression of p300 in macrophages. Our findings elucidate a new molecular mechanism for the anti-inflammatory effect of BBR via the p300/p65Lys310 axis. |
| first_indexed | 2024-04-09T13:29:58Z |
| format | Article |
| id | doaj.art-ccd2a91eb85146ab85354a5f41b7c1b5 |
| institution | Directory Open Access Journal |
| issn | 2213-2317 |
| language | English |
| last_indexed | 2024-04-09T13:29:58Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Redox Biology |
| spelling | doaj.art-ccd2a91eb85146ab85354a5f41b7c1b52023-05-10T04:19:11ZengElsevierRedox Biology2213-23172023-06-0162102704Acetylation of p65Lys310 by p300 in macrophages mediates anti-inflammatory property of berberineShuchen Zhang0Pingyuan Xu1Ziwei Zhu2Lingyan Zhou3Jiao Li4Ruonan Zhou5Yue Kan6Yaru Li7Xizhong Yu8Juan Zhao9Yu Jin10Jing Yan11Penghua Fang12Wenbin Shang13Department of Endocrinology, Jiangsu Province Hospital of Chinese Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China; Key Laboratory for Metabolic Diseases in Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China; School of Chinese Medicine, School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, ChinaDepartment of Endocrinology, Jiangsu Province Hospital of Chinese Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China; Key Laboratory for Metabolic Diseases in Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, ChinaDepartment of Endocrinology, Jiangsu Province Hospital of Chinese Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China; Key Laboratory for Metabolic Diseases in Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, ChinaDepartment of Endocrinology, Jiangsu Province Hospital of Chinese Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China; Key Laboratory for Metabolic Diseases in Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, ChinaDepartment of Endocrinology, Jiangsu Province Hospital of Chinese Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, ChinaDepartment of Endocrinology, Jiangsu Province Hospital of Chinese Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China; Key Laboratory for Metabolic Diseases in Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, ChinaDepartment of Endocrinology, Jiangsu Province Hospital of Chinese Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China; Key Laboratory for Metabolic Diseases in Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, ChinaDepartment of Endocrinology, Jiangsu Province Hospital of Chinese Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China; Key Laboratory for Metabolic Diseases in Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, ChinaKey Laboratory for Metabolic Diseases in Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, ChinaKey Laboratory for Metabolic Diseases in Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, ChinaKey Laboratory for Metabolic Diseases in Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, ChinaKey Laboratory for Metabolic Diseases in Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, ChinaKey Laboratory for Metabolic Diseases in Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China; Corresponding author.Department of Endocrinology, Jiangsu Province Hospital of Chinese Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China; Key Laboratory for Metabolic Diseases in Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China; Corresponding author. Department of Endocrinology, Jiangsu Province Hospital of Chinese Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China.Nuclear factor (NF)-κB plays a pivotal role in the regulation of inflammatory response in macrophages. Berberine (BBR), which is an active constituent isolated from Coptis rhizome, possesses a prominent anti-inflammatory activity. Here we show that BBR changes the global acetylation landscape in LPS-induced protein acetylation of macrophages and reduces the acetylation of NF-κB subunit p65 at site Lys310(p65Lys310), leading to the inhibition of NF-κB translocation and transcriptional activity to suppress the expressions of inflammatory factors. BBR resists the inflammatory response in acute LPS-stimulated mice through downregulation of p65Lys310 acetylation in peritoneal macrophages. In obese mice, BBR alleviates the metabolic disorder and inflammation with the reduced acetylation of p65Lys310 in white adipose tissue. Furthermore, we demonstrate that BBR acts as a regulator of p65Lys310 by inhibiting the expression of p300 in macrophages. Our findings elucidate a new molecular mechanism for the anti-inflammatory effect of BBR via the p300/p65Lys310 axis.http://www.sciencedirect.com/science/article/pii/S2213231723001052Berberinep65p300Inflammatory responseMacrophages |
| spellingShingle | Shuchen Zhang Pingyuan Xu Ziwei Zhu Lingyan Zhou Jiao Li Ruonan Zhou Yue Kan Yaru Li Xizhong Yu Juan Zhao Yu Jin Jing Yan Penghua Fang Wenbin Shang Acetylation of p65Lys310 by p300 in macrophages mediates anti-inflammatory property of berberine Redox Biology Berberine p65 p300 Inflammatory response Macrophages |
| title | Acetylation of p65Lys310 by p300 in macrophages mediates anti-inflammatory property of berberine |
| title_full | Acetylation of p65Lys310 by p300 in macrophages mediates anti-inflammatory property of berberine |
| title_fullStr | Acetylation of p65Lys310 by p300 in macrophages mediates anti-inflammatory property of berberine |
| title_full_unstemmed | Acetylation of p65Lys310 by p300 in macrophages mediates anti-inflammatory property of berberine |
| title_short | Acetylation of p65Lys310 by p300 in macrophages mediates anti-inflammatory property of berberine |
| title_sort | acetylation of p65lys310 by p300 in macrophages mediates anti inflammatory property of berberine |
| topic | Berberine p65 p300 Inflammatory response Macrophages |
| url | http://www.sciencedirect.com/science/article/pii/S2213231723001052 |
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