Optimization of Blood Handling and Peripheral Blood Mononuclear Cell Cryopreservation of Low Cell Number Samples

Optimization of Blood Handling and Peripheral Blood Mononuclear Cell Cryopreservation of Low Cell Number Samples

Background: Rural/remote blood collection can cause delays in processing, reducing PBMC number, viability, cell composition and function. To mitigate these impacts, blood was stored at 4 °C prior to processing. Viable cell number, viability, immune phenotype, and Interferon-γ (IFN-γ) release were me...

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Bibliographic Details
Main Authors: Christopher M. Hope, Dao Huynh, Ying Ying Wong, Helena Oakey, Griffith Boord Perkins, Trung Nguyen, Sabrina Binkowski, Minh Bui, Ace Y. L. Choo, Emily Gibson, Dexing Huang, Ki Wook Kim, Katrina Ngui, William D. Rawlinson, Timothy Sadlon, Jennifer J. Couper, Megan A. S. Penno, Simon C. Barry, on behalf of the ENDIA Study Group
Format: Article
Language:English
Published: MDPI AG 2021-08-01
Series:International Journal of Molecular Sciences
Subjects:
PBMC
blood handling
delay in processing
cryopreservation
cell concentration
Online Access:https://www.mdpi.com/1422-0067/22/17/9129

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https://www.mdpi.com/1422-0067/22/17/9129

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