Manipulating mRNA-binding protein Cth2 function in budding yeast Saccharomyces cerevisiae

Manipulating mRNA-binding protein Cth2 function in budding yeast Saccharomyces cerevisiae

Summary: Here, we present a protocol for modulating the function of the Cth2 mRNA-binding protein (RBP) in Saccharomyces cerevisiae. We describe steps to amplify and integrate mutations in Cth2 that affect its stability and function. Next, we detail the functional assay to verify the activity of the...

Full description

Bibliographic Details
Main Authors: Praveen K. Patnaik, Hanna Barlit, Vyacheslav M. Labunskyy
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Cell-based Assays
Model Organisms
Molecular Biology
Gene Expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723007748

Internet

http://www.sciencedirect.com/science/article/pii/S2666166723007748

Similar Items