Human Telomeres Are Tethered to the Nuclear Envelope during Postmitotic Nuclear Assembly
Telomeres are essential for nuclear organization in yeast and during meiosis in mice. Exploring telomere dynamics in living human cells by advanced time-lapse confocal microscopy allowed us to evaluate the spatial distribution of telomeres within the nuclear volume. We discovered an unambiguous enri...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2012-12-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124712004160 |
| _version_ | 1818368848247652352 |
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| author | Laure Crabbe Anthony J. Cesare James M. Kasuboski James A.J. Fitzpatrick Jan Karlseder |
| author_facet | Laure Crabbe Anthony J. Cesare James M. Kasuboski James A.J. Fitzpatrick Jan Karlseder |
| author_sort | Laure Crabbe |
| collection | DOAJ |
| description | Telomeres are essential for nuclear organization in yeast and during meiosis in mice. Exploring telomere dynamics in living human cells by advanced time-lapse confocal microscopy allowed us to evaluate the spatial distribution of telomeres within the nuclear volume. We discovered an unambiguous enrichment of telomeres at the nuclear periphery during postmitotic nuclear assembly, whereas telomeres were localized more internally during the rest of the cell cycle. Telomere enrichment at the nuclear rim was mediated by physical tethering of telomeres to the nuclear envelope, most likely via specific interactions between the shelterin subunit RAP1 and the nuclear envelope protein Sun1. Genetic interference revealed a critical role in cell-cycle progression for Sun1 but no effect on telomere positioning for RAP1. Our results shed light on the dynamic relocalization of human telomeres during the cell cycle and suggest redundant pathways for tethering telomeres to the nuclear envelope. |
| first_indexed | 2024-12-13T23:14:28Z |
| format | Article |
| id | doaj.art-cd076ee6d9f24865a08d5042f77f54e8 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-13T23:14:28Z |
| publishDate | 2012-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-cd076ee6d9f24865a08d5042f77f54e82022-12-21T23:27:59ZengElsevierCell Reports2211-12472012-12-01261521152910.1016/j.celrep.2012.11.019Human Telomeres Are Tethered to the Nuclear Envelope during Postmitotic Nuclear AssemblyLaure Crabbe0Anthony J. Cesare1James M. Kasuboski2James A.J. Fitzpatrick3Jan Karlseder4Molecular and Cellular Biology Department, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USAMolecular and Cellular Biology Department, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USAWaitt Advanced Biophotonics Center, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USAWaitt Advanced Biophotonics Center, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USAMolecular and Cellular Biology Department, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USATelomeres are essential for nuclear organization in yeast and during meiosis in mice. Exploring telomere dynamics in living human cells by advanced time-lapse confocal microscopy allowed us to evaluate the spatial distribution of telomeres within the nuclear volume. We discovered an unambiguous enrichment of telomeres at the nuclear periphery during postmitotic nuclear assembly, whereas telomeres were localized more internally during the rest of the cell cycle. Telomere enrichment at the nuclear rim was mediated by physical tethering of telomeres to the nuclear envelope, most likely via specific interactions between the shelterin subunit RAP1 and the nuclear envelope protein Sun1. Genetic interference revealed a critical role in cell-cycle progression for Sun1 but no effect on telomere positioning for RAP1. Our results shed light on the dynamic relocalization of human telomeres during the cell cycle and suggest redundant pathways for tethering telomeres to the nuclear envelope.http://www.sciencedirect.com/science/article/pii/S2211124712004160 |
| spellingShingle | Laure Crabbe Anthony J. Cesare James M. Kasuboski James A.J. Fitzpatrick Jan Karlseder Human Telomeres Are Tethered to the Nuclear Envelope during Postmitotic Nuclear Assembly Cell Reports |
| title | Human Telomeres Are Tethered to the Nuclear Envelope during Postmitotic Nuclear Assembly |
| title_full | Human Telomeres Are Tethered to the Nuclear Envelope during Postmitotic Nuclear Assembly |
| title_fullStr | Human Telomeres Are Tethered to the Nuclear Envelope during Postmitotic Nuclear Assembly |
| title_full_unstemmed | Human Telomeres Are Tethered to the Nuclear Envelope during Postmitotic Nuclear Assembly |
| title_short | Human Telomeres Are Tethered to the Nuclear Envelope during Postmitotic Nuclear Assembly |
| title_sort | human telomeres are tethered to the nuclear envelope during postmitotic nuclear assembly |
| url | http://www.sciencedirect.com/science/article/pii/S2211124712004160 |
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