Protocol to visualize trans-interaction of clustered protocadherin using cIPAD, a fluorescent indicator, in cultured human cells and mouse neurons

Protocol to visualize trans-interaction of clustered protocadherin using cIPAD, a fluorescent indicator, in cultured human cells and mouse neurons

Summary: cIPAD is a fluorescent indicator that allows the visualization of trans-interactions of clustered protocadherin (Pcdh), a cell adhesion molecule that mediates neuronal self-recognition. We describe steps for using HEK293T cells to visualize Pcdh trans-interactions across cells as a prelimin...

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Bibliographic Details
Main Authors: Takashi Kanadome, Natsumi Hoshino, Takeharu Nagai, Takeshi Yagi, Tomoki Matsuda
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Biotechnology and bioengineering
Cell Biology
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724000091

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http://www.sciencedirect.com/science/article/pii/S2666166724000091

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