Volume-Associated Clinical and Histopathological Effects of Intranasal Instillation in Syrian Hamsters: Considerations for Infection and Therapeutic Studies
Syrian hamsters are a key animal model of SARS-CoV-2 and other respiratory viruses and are useful for the evaluation of associated medical countermeasures. Delivery of an infectious agent or intervention to the respiratory tract mirrors natural routes of exposure and allows for the evaluation of cli...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/8/898 |
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| author | Catalina Forero Jana M. Ritter Josilene Nascimento Seixas JoAnn D. Coleman-McCray Marie Brake Jillian A. Condrey Cassandra Tansey Stephen R. Welch Sarah C. Genzer Jessica R. Spengler |
| author_facet | Catalina Forero Jana M. Ritter Josilene Nascimento Seixas JoAnn D. Coleman-McCray Marie Brake Jillian A. Condrey Cassandra Tansey Stephen R. Welch Sarah C. Genzer Jessica R. Spengler |
| author_sort | Catalina Forero |
| collection | DOAJ |
| description | Syrian hamsters are a key animal model of SARS-CoV-2 and other respiratory viruses and are useful for the evaluation of associated medical countermeasures. Delivery of an infectious agent or intervention to the respiratory tract mirrors natural routes of exposure and allows for the evaluation of clinically relevant therapeutic administration. The data to support instillation or inoculation volumes are important both for optimal experimental design and to minimize or avoid effects of diluent alone, which may compromise both data interpretation and animal welfare. Here we investigate four intranasal (IN) instillation volumes in hamsters (50, 100, 200, or 400 µL). The animals were monitored daily, and a subset were serially euthanized at one of four pre-determined time-points (1, 3, 7, and 14 days post-instillation). Weight, temperature, oxygen saturation, CBC, radiographs, and respiratory tissue histopathology were assessed to determine changes associated with instillation volume alone. With all the delivery volumes, we found no notable differences between instilled and non-instilled controls in all of the parameters assessed, except for histopathology. In the animals instilled with 200 or 400 µL, inflammation associated with foreign material was detected in the lower respiratory tract indicating that higher volumes may result in aspiration of nasal and/or oropharyngeal material in a subset of animals, resulting in IN instillation-associated histopathology. |
| first_indexed | 2024-03-09T03:59:51Z |
| format | Article |
| id | doaj.art-cd1c7b1c153e4adab016eb70acfb08e1 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T03:59:51Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-cd1c7b1c153e4adab016eb70acfb08e12023-12-03T14:15:33ZengMDPI AGPathogens2076-08172022-08-0111889810.3390/pathogens11080898Volume-Associated Clinical and Histopathological Effects of Intranasal Instillation in Syrian Hamsters: Considerations for Infection and Therapeutic StudiesCatalina Forero0Jana M. Ritter1Josilene Nascimento Seixas2JoAnn D. Coleman-McCray3Marie Brake4Jillian A. Condrey5Cassandra Tansey6Stephen R. Welch7Sarah C. Genzer8Jessica R. Spengler9Comparative Medicine Branch, Division of Scientific Resources, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAInfectious Diseases Pathology Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAInfectious Diseases Pathology Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAViral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAComparative Medicine Branch, Division of Scientific Resources, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAComparative Medicine Branch, Division of Scientific Resources, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAComparative Medicine Branch, Division of Scientific Resources, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAViral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAViral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAViral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USASyrian hamsters are a key animal model of SARS-CoV-2 and other respiratory viruses and are useful for the evaluation of associated medical countermeasures. Delivery of an infectious agent or intervention to the respiratory tract mirrors natural routes of exposure and allows for the evaluation of clinically relevant therapeutic administration. The data to support instillation or inoculation volumes are important both for optimal experimental design and to minimize or avoid effects of diluent alone, which may compromise both data interpretation and animal welfare. Here we investigate four intranasal (IN) instillation volumes in hamsters (50, 100, 200, or 400 µL). The animals were monitored daily, and a subset were serially euthanized at one of four pre-determined time-points (1, 3, 7, and 14 days post-instillation). Weight, temperature, oxygen saturation, CBC, radiographs, and respiratory tissue histopathology were assessed to determine changes associated with instillation volume alone. With all the delivery volumes, we found no notable differences between instilled and non-instilled controls in all of the parameters assessed, except for histopathology. In the animals instilled with 200 or 400 µL, inflammation associated with foreign material was detected in the lower respiratory tract indicating that higher volumes may result in aspiration of nasal and/or oropharyngeal material in a subset of animals, resulting in IN instillation-associated histopathology.https://www.mdpi.com/2076-0817/11/8/898Syrian hamsteranimal modelrespiratory virus modelintranasal instillationintranasal inoculationhistopathology |
| spellingShingle | Catalina Forero Jana M. Ritter Josilene Nascimento Seixas JoAnn D. Coleman-McCray Marie Brake Jillian A. Condrey Cassandra Tansey Stephen R. Welch Sarah C. Genzer Jessica R. Spengler Volume-Associated Clinical and Histopathological Effects of Intranasal Instillation in Syrian Hamsters: Considerations for Infection and Therapeutic Studies Pathogens Syrian hamster animal model respiratory virus model intranasal instillation intranasal inoculation histopathology |
| title | Volume-Associated Clinical and Histopathological Effects of Intranasal Instillation in Syrian Hamsters: Considerations for Infection and Therapeutic Studies |
| title_full | Volume-Associated Clinical and Histopathological Effects of Intranasal Instillation in Syrian Hamsters: Considerations for Infection and Therapeutic Studies |
| title_fullStr | Volume-Associated Clinical and Histopathological Effects of Intranasal Instillation in Syrian Hamsters: Considerations for Infection and Therapeutic Studies |
| title_full_unstemmed | Volume-Associated Clinical and Histopathological Effects of Intranasal Instillation in Syrian Hamsters: Considerations for Infection and Therapeutic Studies |
| title_short | Volume-Associated Clinical and Histopathological Effects of Intranasal Instillation in Syrian Hamsters: Considerations for Infection and Therapeutic Studies |
| title_sort | volume associated clinical and histopathological effects of intranasal instillation in syrian hamsters considerations for infection and therapeutic studies |
| topic | Syrian hamster animal model respiratory virus model intranasal instillation intranasal inoculation histopathology |
| url | https://www.mdpi.com/2076-0817/11/8/898 |
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