Determination of Sterols in Dairy Products and Vegetable Fats by HPLC and GC Methods
Cholesterol concentrations in goat milk, goat milk cheeses, ewe's milk, ewes milk cheeses, dairy bioproducts, and concentrations of cholesterol, stigmasterol and sitosterol in butter, butter with added vegetable fats and margarines were evaluated by RP HPLC method. Parallel analyses by capillar...
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| Language: | English |
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Czech Academy of Agricultural Sciences
2009-06-01
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| Series: | Czech Journal of Food Sciences |
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| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-200910-0042_determination-of-sterols-in-dairy-products-and-vegetable-fats-by-hplc-and-gc-methods.php |
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| author | I. Borkovcová E. Janoušková M. Dračková B. Janštová L. Vorlová |
| author_facet | I. Borkovcová E. Janoušková M. Dračková B. Janštová L. Vorlová |
| author_sort | I. Borkovcová |
| collection | DOAJ |
| description | Cholesterol concentrations in goat milk, goat milk cheeses, ewe's milk, ewes milk cheeses, dairy bioproducts, and concentrations of cholesterol, stigmasterol and sitosterol in butter, butter with added vegetable fats and margarines were evaluated by RP HPLC method. Parallel analyses by capillary GC were performed. Prior to the final chromatographic analyses the saponification step was used, followed by the extraction of the unsaponificable residue into n-hexane. Parameters of RP HPLC method were compared with parameters of GC determination. The detection limits (LOD) determinated on the bases of blank samples analysis were 5.2 mg/kg for cholesterol, 4.8 mg/kg for stigmasterol and 14.7 mg/kg for sitosterol. Recovery ranged between 80-92%, repeatibility expressed as RSD of 12 parallel samples measurements was 4.2-6.8%. Accuracy tested on the SRM 1845 Whole Egg Powder (NIST) was 95.7%. |
| first_indexed | 2024-04-10T08:35:17Z |
| format | Article |
| id | doaj.art-cd1f6e3372df45c08e4e1f7fc0e91741 |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:35:17Z |
| publishDate | 2009-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-cd1f6e3372df45c08e4e1f7fc0e917412023-02-23T03:27:31ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172009-06-0127Special Issue 1S217S21910.17221/1073-CJFScjf-200910-0042Determination of Sterols in Dairy Products and Vegetable Fats by HPLC and GC MethodsI. BorkovcováE. Janoušková0M. Dračková1B. Janštová2L. Vorlová3Department of Milk Hygiene and Technology, University of Veterinary and Pharmaceutical Sciences in Brno, 612 42 Brno, Czech Republic, *E-mail: borkovcovai@vfu.czDepartment of Milk Hygiene and Technology, University of Veterinary and Pharmaceutical Sciences in Brno, 612 42 Brno, Czech Republic, *E-mail: borkovcovai@vfu.czDepartment of Milk Hygiene and Technology, University of Veterinary and Pharmaceutical Sciences in Brno, 612 42 Brno, Czech Republic, *E-mail: borkovcovai@vfu.czDepartment of Milk Hygiene and Technology, University of Veterinary and Pharmaceutical Sciences in Brno, 612 42 Brno, Czech Republic, *E-mail: borkovcovai@vfu.czCholesterol concentrations in goat milk, goat milk cheeses, ewe's milk, ewes milk cheeses, dairy bioproducts, and concentrations of cholesterol, stigmasterol and sitosterol in butter, butter with added vegetable fats and margarines were evaluated by RP HPLC method. Parallel analyses by capillary GC were performed. Prior to the final chromatographic analyses the saponification step was used, followed by the extraction of the unsaponificable residue into n-hexane. Parameters of RP HPLC method were compared with parameters of GC determination. The detection limits (LOD) determinated on the bases of blank samples analysis were 5.2 mg/kg for cholesterol, 4.8 mg/kg for stigmasterol and 14.7 mg/kg for sitosterol. Recovery ranged between 80-92%, repeatibility expressed as RSD of 12 parallel samples measurements was 4.2-6.8%. Accuracy tested on the SRM 1845 Whole Egg Powder (NIST) was 95.7%.https://cjfs.agriculturejournals.cz/artkey/cjf-200910-0042_determination-of-sterols-in-dairy-products-and-vegetable-fats-by-hplc-and-gc-methods.phpcholesterolsterolsmilkdairy productsrp hplcgc |
| spellingShingle | I. Borkovcová E. Janoušková M. Dračková B. Janštová L. Vorlová Determination of Sterols in Dairy Products and Vegetable Fats by HPLC and GC Methods Czech Journal of Food Sciences cholesterol sterols milk dairy products rp hplc gc |
| title | Determination of Sterols in Dairy Products and Vegetable Fats by HPLC and GC Methods |
| title_full | Determination of Sterols in Dairy Products and Vegetable Fats by HPLC and GC Methods |
| title_fullStr | Determination of Sterols in Dairy Products and Vegetable Fats by HPLC and GC Methods |
| title_full_unstemmed | Determination of Sterols in Dairy Products and Vegetable Fats by HPLC and GC Methods |
| title_short | Determination of Sterols in Dairy Products and Vegetable Fats by HPLC and GC Methods |
| title_sort | determination of sterols in dairy products and vegetable fats by hplc and gc methods |
| topic | cholesterol sterols milk dairy products rp hplc gc |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-200910-0042_determination-of-sterols-in-dairy-products-and-vegetable-fats-by-hplc-and-gc-methods.php |
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