Improving saliva shotgun metagenomics by chemical host DNA depletion

Improving saliva shotgun metagenomics by chemical host DNA depletion

Abstract Background Shotgun sequencing of microbial communities provides in-depth knowledge of the microbiome by cataloging bacterial, fungal, and viral gene content within a sample, providing an advantage over amplicon sequencing approaches that assess taxonomy but not function and are taxonomicall...

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Bibliographic Details
Main Authors: Clarisse A. Marotz, Jon G. Sanders, Cristal Zuniga, Livia S. Zaramela, Rob Knight, Karsten Zengler
Format: Article
Language:English
Published: BMC 2018-02-01
Series:Microbiome
Subjects:
Microbiome
Host depletion
Microbial enrichment
Propidium monoazide
Shotgun sequencing
Saliva
Online Access:http://link.springer.com/article/10.1186/s40168-018-0426-3

Internet

http://link.springer.com/article/10.1186/s40168-018-0426-3

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