Protocol for detecting macrophage-mediated cancer cell phagocytosis in vitro and in vivo

Summary: Here, we present optimized approaches to identify the efficiency of cancer cell phagocytosis by macrophages in vitro and in vivo. We describe the preparation and co-culture of macrophages and cancer cells, followed by in vitro phagocytosis assay using flow cytometry and confocal microscopy,...

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Bibliographic Details
Main Authors: Chuan Xu, Hong Wu, Yiqiang Liu, Feifei Li, Rajesh Kumar Manne, Hui-Kuan Lin
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722008206