Impact of Preservation Method and 16S rRNA Hypervariable Region on Gut Microbiota Profiling

Impact of Preservation Method and 16S rRNA Hypervariable Region on Gut Microbiota Profiling

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ABSTRACT Proper preservation of stool samples to minimize microbial community shifts and inactivate infectious agents is important for self-collected specimens requiring shipment to laboratories when cold chain transport is not feasible. In this study, we evaluated the performance of six preservatio...

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Bibliographic Details
Main Authors: Zigui Chen, Pak Chun Hui, Mamie Hui, Yun Kit Yeoh, Po Yee Wong, Martin C. W. Chan, Martin C. S. Wong, Siew C. Ng, Francis K. L. Chan, Paul K. S. Chan
Format: Article
Language:English
Published: American Society for Microbiology 2019-02-01
Series:mSystems
Subjects:
16S rRNA gene
amplicon sequencing
bacterial culture
gut microbiota
preservative
Online Access:https://journals.asm.org/doi/10.1128/msystems.00271-18

Internet

https://journals.asm.org/doi/10.1128/msystems.00271-18

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