A case of 5-fluorouracil toxicity
Dihydrouracil (UH2)/Uracil (U) ratio in plasma was determinated as a surrogate marker for dihydropyrimidine dehydrogenase (DPD) activity. The purpose of the study was to investigate the ralationship between the UH2/U plasma ratio and the variation of DPD gene (DPYD), associated with a deficiency of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2011-01-01
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| Series: | Journal of Biological Research |
| Online Access: | http://www.pagepressjournals.org/index.php/jbr/article/view/4648 |
| Summary: | Dihydrouracil (UH2)/Uracil (U) ratio in plasma was determinated as a surrogate marker for dihydropyrimidine dehydrogenase (DPD) activity. The purpose of the study was to investigate the ralationship between the UH2/U plasma ratio and the variation of DPD gene (DPYD), associated with a deficiency of DPD activity, in a patient who developed a severe adverse reaction to 5-fluorouracil. Patient's plasma smaple and those of 20 healthy volunteers, used as control, were analyzed. The plasma concentrations of UH2 and U were assayed by HPLC-UV. UH2/U plasma ratio of the patient was 4.31; the mean ± SD of UH2/U plasma ratios in controls was 5.26 ± 2.08. The UH2/U ratio of the patient was lower than the mean values recorded from a reference population suggesting a reduction of DPD activity according to haplotype of the patient, previously identified. |
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| ISSN: | 1826-8838 2284-0230 |