Spatially coherent diffusion of human RNA Pol II depends on transcriptional state rather than chromatin motion
Gene transcription by RNA polymerase II (RNAPol II) is a tightly regulated process in the genomic, temporal, and spatial context. Recently, we have shown that chromatin exhibits spatially coherently moving regions over the entire nucleus, which is enhanced by transcription. Yet, it remains unclear h...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Nucleus |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19491034.2022.2088988 |
| _version_ | 1811242455704535040 |
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| author | Roman Barth Haitham A. Shaban |
| author_facet | Roman Barth Haitham A. Shaban |
| author_sort | Roman Barth |
| collection | DOAJ |
| description | Gene transcription by RNA polymerase II (RNAPol II) is a tightly regulated process in the genomic, temporal, and spatial context. Recently, we have shown that chromatin exhibits spatially coherently moving regions over the entire nucleus, which is enhanced by transcription. Yet, it remains unclear how the mobility of RNA Pol II molecules is affected by transcription regulation and whether this response depends on the coordinated chromatin movement. We applied our Dense Flow reConstruction and Correlation method to analyze nucleus-wide coherent movements of RNA Pol II in living human cancer cells. We observe a spatially coherent movement of RNA Pol II molecules over [Formula: see text]1 μm, which depends on transcriptional activity. Inducing transcription in quiescent cells decreased the coherent motion of RNA Pol II. We then quantify the spatial correlation length of RNA Pol II in the context of DNA motion. RNA Pol II and chromatin spatially coherent motions respond oppositely to transcriptional activities. Our study holds the potential of studying the chromatin environment in different nuclear processes. |
| first_indexed | 2024-04-12T13:51:32Z |
| format | Article |
| id | doaj.art-cdbce1d12381422cbfe393ff83002b11 |
| institution | Directory Open Access Journal |
| issn | 1949-1034 1949-1042 |
| language | English |
| last_indexed | 2024-04-12T13:51:32Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Nucleus |
| spelling | doaj.art-cdbce1d12381422cbfe393ff83002b112022-12-22T03:30:30ZengTaylor & Francis GroupNucleus1949-10341949-10422022-12-0113119420210.1080/19491034.2022.2088988Spatially coherent diffusion of human RNA Pol II depends on transcriptional state rather than chromatin motionRoman Barth0Haitham A. Shaban1Department of Bionanoscience, Delft University of Technology, CJ Delft, The NetherlandsSpectroscopy Department, Physics Division, National Research Centre, Dokki, EgyptGene transcription by RNA polymerase II (RNAPol II) is a tightly regulated process in the genomic, temporal, and spatial context. Recently, we have shown that chromatin exhibits spatially coherently moving regions over the entire nucleus, which is enhanced by transcription. Yet, it remains unclear how the mobility of RNA Pol II molecules is affected by transcription regulation and whether this response depends on the coordinated chromatin movement. We applied our Dense Flow reConstruction and Correlation method to analyze nucleus-wide coherent movements of RNA Pol II in living human cancer cells. We observe a spatially coherent movement of RNA Pol II molecules over [Formula: see text]1 μm, which depends on transcriptional activity. Inducing transcription in quiescent cells decreased the coherent motion of RNA Pol II. We then quantify the spatial correlation length of RNA Pol II in the context of DNA motion. RNA Pol II and chromatin spatially coherent motions respond oppositely to transcriptional activities. Our study holds the potential of studying the chromatin environment in different nuclear processes.https://www.tandfonline.com/doi/10.1080/19491034.2022.2088988Transcriptional regulationquantitative imaging of RNA Pol IILive-cell imagingchromatin dynamicsgenome organization |
| spellingShingle | Roman Barth Haitham A. Shaban Spatially coherent diffusion of human RNA Pol II depends on transcriptional state rather than chromatin motion Nucleus Transcriptional regulation quantitative imaging of RNA Pol II Live-cell imaging chromatin dynamics genome organization |
| title | Spatially coherent diffusion of human RNA Pol II depends on transcriptional state rather than chromatin motion |
| title_full | Spatially coherent diffusion of human RNA Pol II depends on transcriptional state rather than chromatin motion |
| title_fullStr | Spatially coherent diffusion of human RNA Pol II depends on transcriptional state rather than chromatin motion |
| title_full_unstemmed | Spatially coherent diffusion of human RNA Pol II depends on transcriptional state rather than chromatin motion |
| title_short | Spatially coherent diffusion of human RNA Pol II depends on transcriptional state rather than chromatin motion |
| title_sort | spatially coherent diffusion of human rna pol ii depends on transcriptional state rather than chromatin motion |
| topic | Transcriptional regulation quantitative imaging of RNA Pol II Live-cell imaging chromatin dynamics genome organization |
| url | https://www.tandfonline.com/doi/10.1080/19491034.2022.2088988 |
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