Harmonic Generation Microscopy 2.0: New Tricks Empowering Intravital Imaging for Neuroscience
Optical harmonic generation, e.g., second- (SHG) and third-harmonic generation (THG), provides intrinsic contrasts for three-dimensional intravital microscopy. Contrary to two-photon excited fluorescence (TPEF), however, they have found relatively specialized applications, such as imaging collagenou...
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Format: | Article |
Language: | English |
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Frontiers Media S.A.
2019-10-01
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Series: | Frontiers in Molecular Biosciences |
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Online Access: | https://www.frontiersin.org/article/10.3389/fmolb.2019.00099/full |