Harmonic Generation Microscopy 2.0: New Tricks Empowering Intravital Imaging for Neuroscience
Optical harmonic generation, e.g., second- (SHG) and third-harmonic generation (THG), provides intrinsic contrasts for three-dimensional intravital microscopy. Contrary to two-photon excited fluorescence (TPEF), however, they have found relatively specialized applications, such as imaging collagenou...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmolb.2019.00099/full |
| _version_ | 1811337158962708480 |
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| author | Hyungsik Lim |
| author_facet | Hyungsik Lim |
| author_sort | Hyungsik Lim |
| collection | DOAJ |
| description | Optical harmonic generation, e.g., second- (SHG) and third-harmonic generation (THG), provides intrinsic contrasts for three-dimensional intravital microscopy. Contrary to two-photon excited fluorescence (TPEF), however, they have found relatively specialized applications, such as imaging collagenous and non-specific tissues, respectively. Here we review recent advances that broaden the capacity of SHG and THG for imaging the central nervous system in particular. The fundamental contrast mechanisms are reviewed as they encode novel information including molecular origin, spectroscopy, functional probes, and image analysis, which lay foundations for promising future applications in neuroscience. |
| first_indexed | 2024-04-13T17:50:50Z |
| format | Article |
| id | doaj.art-cdbfa6e650da422bb7c26af532bcfd03 |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-04-13T17:50:50Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-cdbfa6e650da422bb7c26af532bcfd032022-12-22T02:36:44ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2019-10-01610.3389/fmolb.2019.00099482057Harmonic Generation Microscopy 2.0: New Tricks Empowering Intravital Imaging for NeuroscienceHyungsik LimOptical harmonic generation, e.g., second- (SHG) and third-harmonic generation (THG), provides intrinsic contrasts for three-dimensional intravital microscopy. Contrary to two-photon excited fluorescence (TPEF), however, they have found relatively specialized applications, such as imaging collagenous and non-specific tissues, respectively. Here we review recent advances that broaden the capacity of SHG and THG for imaging the central nervous system in particular. The fundamental contrast mechanisms are reviewed as they encode novel information including molecular origin, spectroscopy, functional probes, and image analysis, which lay foundations for promising future applications in neuroscience.https://www.frontiersin.org/article/10.3389/fmolb.2019.00099/fullintravital imagingsecond-harmonic generationthird-harmonic generationnon-linear optical microscopylabel-free imaging |
| spellingShingle | Hyungsik Lim Harmonic Generation Microscopy 2.0: New Tricks Empowering Intravital Imaging for Neuroscience Frontiers in Molecular Biosciences intravital imaging second-harmonic generation third-harmonic generation non-linear optical microscopy label-free imaging |
| title | Harmonic Generation Microscopy 2.0: New Tricks Empowering Intravital Imaging for Neuroscience |
| title_full | Harmonic Generation Microscopy 2.0: New Tricks Empowering Intravital Imaging for Neuroscience |
| title_fullStr | Harmonic Generation Microscopy 2.0: New Tricks Empowering Intravital Imaging for Neuroscience |
| title_full_unstemmed | Harmonic Generation Microscopy 2.0: New Tricks Empowering Intravital Imaging for Neuroscience |
| title_short | Harmonic Generation Microscopy 2.0: New Tricks Empowering Intravital Imaging for Neuroscience |
| title_sort | harmonic generation microscopy 2 0 new tricks empowering intravital imaging for neuroscience |
| topic | intravital imaging second-harmonic generation third-harmonic generation non-linear optical microscopy label-free imaging |
| url | https://www.frontiersin.org/article/10.3389/fmolb.2019.00099/full |
| work_keys_str_mv | AT hyungsiklim harmonicgenerationmicroscopy20newtricksempoweringintravitalimagingforneuroscience |