Automated 16S Sequencing Using an R-Based Analysis Module for Bacterial Identification

Automated 16S Sequencing Using an R-Based Analysis Module for Bacterial Identification

ABSTRACT Sanger sequencing of the 16S rRNA gene is routinely used for the identification of bacterial isolates. However, this method is still performed mostly in more-specialized reference laboratories, and traditional protocols can be labor intensive. In this study, 99 clinical bacterial isolates w...

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Bibliographic Details
Main Authors: Kerstin Locher, Corrie R. Belanger, Eric Eckbo, Melissa Caza, Billie Velapatino, Marthe K. Charles
Format: Article
Language:English
Published: American Society for Microbiology 2022-04-01
Series:Microbiology Spectrum
Subjects:
16S Sanger sequencing
automated workflow
bacterial identification
16S RNA
R script
automation
Online Access:https://journals.asm.org/doi/10.1128/spectrum.00408-22

Internet

https://journals.asm.org/doi/10.1128/spectrum.00408-22

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