New protocol for kinetic assay seeding ability recovery “KASAR” from formalin-fixed paraffin-embedded tissues

New protocol for kinetic assay seeding ability recovery “KASAR” from formalin-fixed paraffin-embedded tissues

The real-time quaking-induced conversion (RT-QuIC) alpha-synuclein (aSyn) protein kinetic seeding assay has been very useful for detecting pathological aggregates in various synucleinopathies including Parkinson’s disease (PD). This biomarker assay relies on fresh frozen tissue to effectively seed a...

पूर्ण विवरण

ग्रंथसूची विवरण
मुख्य लेखकों:	Monica Hepker, Griffin Clabaugh, Huajun Jin, Anumantha G. Kanthasamy
स्वरूप:	लेख
भाषा:	English
प्रकाशित:	Frontiers Media S.A. 2023-01-01
श्रृंखला:	Frontiers in Molecular Biosciences
विषय:	biomarker RT-QuIC formalin-fixed kinetic assay alpha-synuclein protein aggregation
ऑनलाइन पहुंच:	https://www.frontiersin.org/articles/10.3389/fmolb.2023.1087982/full

समान संसाधन

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Alpha-synuclein seeding shows a wide heterogeneity in multiple system atrophy
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Are We Ready for Detecting α-Synuclein Prone to Aggregation in Patients? The Case of “Protein-Misfolding Cyclic Amplification” and “Real-Time Quaking-Induced Conversion” as Diagnostic Tools
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Defining the Protein Seeds of Neurodegeneration using Real-Time Quaking-Induced Conversion Assays
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Kinetic parameters of alpha-synuclein seed amplification assay correlate with cognitive impairment in patients with Lewy body disorders
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RT-QuIC and Related Assays for Detecting and Quantifying Prion-like Pathological Seeds of α-Synuclein
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प्रकाशित: (2022-04-01)

Cerebrospinal fluid lipoproteins inhibit α-synuclein aggregation by interacting with oligomeric species in seed amplification assays
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Editorial: Utility of protein aggregation assays from laboratory to clinical application
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Efficient RT-QuIC seeding activity for α-synuclein in olfactory mucosa samples of patients with Parkinson’s disease and multiple system atrophy
द्वारा: Chiara Maria Giulia De Luca, और अन्य
प्रकाशित: (2019-08-01)

Evolution of Diagnostic Tests for Chronic Wasting Disease, a Naturally Occurring Prion Disease of Cervids
द्वारा: Nicholas J. Haley, और अन्य
प्रकाशित: (2017-08-01)

Clinical, neuropathological, and molecular characteristics of rapidly progressive dementia with Lewy bodies: a distinct clinicopathological entity?
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प्रकाशित: (2024-09-01)

Prion Seeds Distribute throughout the Eyes of Sporadic Creutzfeldt-Jakob Disease Patients
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RT-QuIC detection of chronic wasting disease prion in platelet samples of white-tailed deer
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Editorial: Molecular determinants of protein assemblies in health and disease, Volume II
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Optimization of RT-QuIC Assay Duration for Screening Chronic Wasting Disease in White-Tailed Deer
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प्रकाशित: (2024-02-01)

The Alpha-Synuclein RT-QuIC Products Generated by the Olfactory Mucosa of Patients with Parkinson’s Disease and Multiple System Atrophy Induce Inflammatory Responses in SH-SY5Y Cells
द्वारा: Chiara Maria Giulia De Luca, और अन्य
प्रकाशित: (2021-12-01)

The Latest Research on RT-QuIC Assays—A Literature Review
द्वारा: Thi-Thu-Trang Dong, और अन्य
प्रकाशित: (2021-03-01)

Essential Components of Synthetic Infectious Prion Formation De Novo
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Effect of the micro-environment on α-synuclein conversion and implication in seeded conversion assays
द्वारा: Niccolo Candelise, और अन्य
प्रकाशित: (2020-01-01)

Pathological α-synuclein detected by real-time quaking-induced conversion in synucleinopathies
द्वारा: Juan Huang, और अन्य
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Clinical application of prion-like seeding in α-synucleinopathies: Early and non-invasive diagnosis and therapeutic development
द्वारा: Jiaqi Li, और अन्य
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Application of α-Syn Real-Time Quaking-Induced Conversion for Brain and Skin Specimens of the Chinese Patients With Parkinson’s Disease
द्वारा: Dong-Dong Chen, और अन्य
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Chronic Wasting Disease: State of the Science
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Validation and Application of Skin RT-QuIC to Patients in China with Probable CJD
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Diagnosing multiple system atrophy: current clinical guidance and emerging molecular biomarkers
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The paraffin-embedded RNA metric (PERM) for RNA isolated from formalin-fixed, paraffin-embedded tissue
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The Effect of Fibrosis on DNA Yield from Formalin Fixed Paraffin Embedded Tissues
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RT-QuIC Using C-Terminally Truncated α-Synuclein Forms Detects Differences in Seeding Propensity of Different Brain Regions from Synucleinopathies
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