Determination of nosiheptide residue in food animal tissues by high performance liquid chromatography-tandem mass spectrometry
ObjectiveTo develop a method for the determination of nosiheptide residue in food animal tissues （porcine muscle， porcine liver， porcine kidney， chicken muscle， chicken liver and chicken kidney） with high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS）.MethodsAfter extracted...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
The Editorial Office of Chinese Journal of Food Hygiene
2022-07-01
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| Series: | Zhongguo shipin weisheng zazhi |
| Subjects: | |
| Online Access: | http://www.zgspws.com/zgspwszz/article/abstract/202204019?st=article_issue |
| Summary: | ObjectiveTo develop a method for the determination of nosiheptide residue in food animal tissues （porcine muscle， porcine liver， porcine kidney， chicken muscle， chicken liver and chicken kidney） with high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS）.MethodsAfter extracted by 0.1% acidic acetonitrile， the nosiheptide extract was cleaned up by Captiva ND cartridge and determined by HPLC-MS/MS in negative electrospray ionization mode. Quantification was performed using corresponding matrix-matched calibration curves.ResultsNosiheptide in corresponding matrix showed good linear relationships （r>0.99） in the calibration range of 2~200 μg/L， and the limit of detection （LOD） and limit of quantification （LOQ） of the method were 2.0 and 7.0 μg/kg， respectively. The recoveries of the method ranged from 76.27% to 92.31% at the spiking level of 3.5， 7.0 and 70 μg/kg with relative standard deviations of 2.15%~8.03%.ConclusionThe proposed method is simple， rapid， accurate as well as reproducible and can be applied to monitor and determine nosiheptide residue in food animal tissues. |
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| ISSN: | 1004-8456 |