Sustainable production with obtaining glucosamine from crab exoskeletons
ABSTRACT: Two chemical treatments, five enzymatic (pectinase, lipase, hemicellulase, hemicellulose-cellulase or lipase-pectinase) and one microbiological (Bacillus subtilis) treatment were evaluated to obtain glucosamine hydrochloride (Gluc-HCl) from the chitin obtained from crab (Callinectes belli...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Santa Maria
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| Series: | Ciência Rural |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000900752&lng=en&tlng=en |
| _version_ | 1811319974925434880 |
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| author | Jorge Montiel-Montoya Maribel Valdez-Morales Cesar Reyes Hebert Jair Barrales-Cureño |
| author_facet | Jorge Montiel-Montoya Maribel Valdez-Morales Cesar Reyes Hebert Jair Barrales-Cureño |
| author_sort | Jorge Montiel-Montoya |
| collection | DOAJ |
| description | ABSTRACT: Two chemical treatments, five enzymatic (pectinase, lipase, hemicellulase, hemicellulose-cellulase or lipase-pectinase) and one microbiological (Bacillus subtilis) treatment were evaluated to obtain glucosamine hydrochloride (Gluc-HCl) from the chitin obtained from crab (Callinectes bellicosus) exoskeletons. Chemical treatments were referred as Method A (HCl hydrolysis during 75 min at 90°C) and Method B (HCl hydrolysis during 20 min and 14 h of rest). Glucosamine and, in some cases, N-acetyl-D-glucosamine were identified and quantified by HPLC. Treatments with the greater concentrations of Gluc-HCl in descending order were: lipase (94.4 mg/g), microbiological (45.7 mg/g), lipase-pectinase (22.9 mg/g), hemicellulase-cellulase (20.9 mg/g), hemicellulase (15.3 mg/g), pectinase (10.7 mg/g), Chemical A (7.3mg/g) and Chemical B (7.3mg/g). In terms of yield, the best treatments in descending order were: pectinase (94%), microbiological (94%), hemicellulase (92%), lipase (91%), Chemical B (88%), lipase-pectinase (88%), hemicellulase-cellulase (86%) and Chemical A (28.5%). The two most profound treatments were lipase and microbiological, so they are proposed as part of a viable method to produce Gluc-HCl from crab exoskeletons; they are ecofriendly procedures and could add value to the crab´s productive chain. |
| first_indexed | 2024-04-13T12:51:34Z |
| format | Article |
| id | doaj.art-cddd9210e24547e780e7c4080d2779b2 |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-04-13T12:51:34Z |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-cddd9210e24547e780e7c4080d2779b22022-12-22T02:46:12ZengUniversidade Federal de Santa MariaCiência Rural1678-459649910.1590/0103-8478cr20190021S0103-84782019000900752Sustainable production with obtaining glucosamine from crab exoskeletonsJorge Montiel-MontoyaMaribel Valdez-MoralesCesar ReyesHebert Jair Barrales-CureñoABSTRACT: Two chemical treatments, five enzymatic (pectinase, lipase, hemicellulase, hemicellulose-cellulase or lipase-pectinase) and one microbiological (Bacillus subtilis) treatment were evaluated to obtain glucosamine hydrochloride (Gluc-HCl) from the chitin obtained from crab (Callinectes bellicosus) exoskeletons. Chemical treatments were referred as Method A (HCl hydrolysis during 75 min at 90°C) and Method B (HCl hydrolysis during 20 min and 14 h of rest). Glucosamine and, in some cases, N-acetyl-D-glucosamine were identified and quantified by HPLC. Treatments with the greater concentrations of Gluc-HCl in descending order were: lipase (94.4 mg/g), microbiological (45.7 mg/g), lipase-pectinase (22.9 mg/g), hemicellulase-cellulase (20.9 mg/g), hemicellulase (15.3 mg/g), pectinase (10.7 mg/g), Chemical A (7.3mg/g) and Chemical B (7.3mg/g). In terms of yield, the best treatments in descending order were: pectinase (94%), microbiological (94%), hemicellulase (92%), lipase (91%), Chemical B (88%), lipase-pectinase (88%), hemicellulase-cellulase (86%) and Chemical A (28.5%). The two most profound treatments were lipase and microbiological, so they are proposed as part of a viable method to produce Gluc-HCl from crab exoskeletons; they are ecofriendly procedures and could add value to the crab´s productive chain.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000900752&lng=en&tlng=enCallinectes bellicosusextraction methodsglucosamine hydrochlorideHPLC analysis |
| spellingShingle | Jorge Montiel-Montoya Maribel Valdez-Morales Cesar Reyes Hebert Jair Barrales-Cureño Sustainable production with obtaining glucosamine from crab exoskeletons Ciência Rural Callinectes bellicosus extraction methods glucosamine hydrochloride HPLC analysis |
| title | Sustainable production with obtaining glucosamine from crab exoskeletons |
| title_full | Sustainable production with obtaining glucosamine from crab exoskeletons |
| title_fullStr | Sustainable production with obtaining glucosamine from crab exoskeletons |
| title_full_unstemmed | Sustainable production with obtaining glucosamine from crab exoskeletons |
| title_short | Sustainable production with obtaining glucosamine from crab exoskeletons |
| title_sort | sustainable production with obtaining glucosamine from crab exoskeletons |
| topic | Callinectes bellicosus extraction methods glucosamine hydrochloride HPLC analysis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000900752&lng=en&tlng=en |
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