Using Microbial Responses Viewer and a Regression Approach to Assess the Effect of pH, Activity of Water and Temperature on the Survival of <i>Campylobacter</i> spp.

This study aimed at developing a model for evaluating the survival of various <i>Campylobacter jejuni</i> strains under different conditions in culture media and poultry data from ComBase. <i>Campylobacter</i> data of culture media (116) and poultry (19) were collected from M...

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Bibliographic Details
Main Authors: Hayrunisa Icen, Maria Rosaria Corbo, Milena Sinigaglia, Burcu Irem Omurtag Korkmaz, Antonio Bevilacqua
Format: Article
Language:English
Published: MDPI AG 2022-02-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/11/5/637
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Summary:This study aimed at developing a model for evaluating the survival of various <i>Campylobacter jejuni</i> strains under different conditions in culture media and poultry data from ComBase. <i>Campylobacter</i> data of culture media (116) and poultry (19) were collected from Microbial Responses Viewer, an additional tool of ComBase. The Weibull equation was selected as a suitable model for the analysis of survival data because of the nonlinearity of survival curves. Then, the fitting parameters (first reduction time and shape parameter) were analysed through a Kruskall–Wallis test and box-whisker plots, thus pointing out the existence of two classes of temperature (0–12 °C and 15–25 °C) and pH (4–6.5 and 7–7.5) acting on the viability of <i>C. jejuni</i>. Finally, a general regression model was used to build a comprehensive function; all factors were significant, but temperature was the most significant variable, followed by pH and water activity. In addition, desirability and prediction profiles highlighted a negative correlation of the first reduction time with temperature and a positive correlation with pH and water activity.
ISSN:2304-8158