Measurement of in vivo cholesterol synthesis from 2H2O: a rapid procedure for the isolation, combustion, and isotopic assay of erythrocyte cholesterol.

A rapid preparative scale purification of erythrocyte free cholesterol has been developed for measurements of in vivo cholesterol synthesis from 2H2O. The quantity and purity of cholesterol obtained is suitable for combustion, zinc reduction of the water formed, and determination of deuterium isotop...

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Bibliographic Details
Main Authors: WW Wong, DL Hachey, A Feste, J Leggitt, LL Clarke, WG Pond, PD Klein
Format: Article
Language:English
Published: Elsevier 1991-06-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520420012