Measurement of in vivo cholesterol synthesis from 2H2O: a rapid procedure for the isolation, combustion, and isotopic assay of erythrocyte cholesterol.
A rapid preparative scale purification of erythrocyte free cholesterol has been developed for measurements of in vivo cholesterol synthesis from 2H2O. The quantity and purity of cholesterol obtained is suitable for combustion, zinc reduction of the water formed, and determination of deuterium isotop...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1991-06-01
|
| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520420012 |
| _version_ | 1818647249326964736 |
|---|---|
| author | WW Wong DL Hachey A Feste J Leggitt LL Clarke WG Pond PD Klein |
| author_facet | WW Wong DL Hachey A Feste J Leggitt LL Clarke WG Pond PD Klein |
| author_sort | WW Wong |
| collection | DOAJ |
| description | A rapid preparative scale purification of erythrocyte free cholesterol has been developed for measurements of in vivo cholesterol synthesis from 2H2O. The quantity and purity of cholesterol obtained is suitable for combustion, zinc reduction of the water formed, and determination of deuterium isotopic content by gas isotope ratio mass spectrometry. The ability to detect and to quantitate a range of cholesterol synthesis rates is illustrated by measurements on young pigs receiving diets without and with added dietary cholesterol. |
| first_indexed | 2024-12-17T00:59:32Z |
| format | Article |
| id | doaj.art-ce03192723584c8ea199d319bed09645 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T00:59:32Z |
| publishDate | 1991-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-ce03192723584c8ea199d319bed096452022-12-21T22:09:31ZengElsevierJournal of Lipid Research0022-22751991-06-0132610491056Measurement of in vivo cholesterol synthesis from 2H2O: a rapid procedure for the isolation, combustion, and isotopic assay of erythrocyte cholesterol.WW Wong0DL Hachey1A Feste2J Leggitt3LL Clarke4WG Pond5PD Klein6Department of Pediatrics, Baylor College of Medicine, Houston, TX.Department of Pediatrics, Baylor College of Medicine, Houston, TX.Department of Pediatrics, Baylor College of Medicine, Houston, TX.Department of Pediatrics, Baylor College of Medicine, Houston, TX.Department of Pediatrics, Baylor College of Medicine, Houston, TX.Department of Pediatrics, Baylor College of Medicine, Houston, TX.Department of Pediatrics, Baylor College of Medicine, Houston, TX.A rapid preparative scale purification of erythrocyte free cholesterol has been developed for measurements of in vivo cholesterol synthesis from 2H2O. The quantity and purity of cholesterol obtained is suitable for combustion, zinc reduction of the water formed, and determination of deuterium isotopic content by gas isotope ratio mass spectrometry. The ability to detect and to quantitate a range of cholesterol synthesis rates is illustrated by measurements on young pigs receiving diets without and with added dietary cholesterol.http://www.sciencedirect.com/science/article/pii/S0022227520420012 |
| spellingShingle | WW Wong DL Hachey A Feste J Leggitt LL Clarke WG Pond PD Klein Measurement of in vivo cholesterol synthesis from 2H2O: a rapid procedure for the isolation, combustion, and isotopic assay of erythrocyte cholesterol. Journal of Lipid Research |
| title | Measurement of in vivo cholesterol synthesis from 2H2O: a rapid procedure for the isolation, combustion, and isotopic assay of erythrocyte cholesterol. |
| title_full | Measurement of in vivo cholesterol synthesis from 2H2O: a rapid procedure for the isolation, combustion, and isotopic assay of erythrocyte cholesterol. |
| title_fullStr | Measurement of in vivo cholesterol synthesis from 2H2O: a rapid procedure for the isolation, combustion, and isotopic assay of erythrocyte cholesterol. |
| title_full_unstemmed | Measurement of in vivo cholesterol synthesis from 2H2O: a rapid procedure for the isolation, combustion, and isotopic assay of erythrocyte cholesterol. |
| title_short | Measurement of in vivo cholesterol synthesis from 2H2O: a rapid procedure for the isolation, combustion, and isotopic assay of erythrocyte cholesterol. |
| title_sort | measurement of in vivo cholesterol synthesis from 2h2o a rapid procedure for the isolation combustion and isotopic assay of erythrocyte cholesterol |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520420012 |
| work_keys_str_mv | AT wwwong measurementofinvivocholesterolsynthesisfrom2h2oarapidprocedurefortheisolationcombustionandisotopicassayoferythrocytecholesterol AT dlhachey measurementofinvivocholesterolsynthesisfrom2h2oarapidprocedurefortheisolationcombustionandisotopicassayoferythrocytecholesterol AT afeste measurementofinvivocholesterolsynthesisfrom2h2oarapidprocedurefortheisolationcombustionandisotopicassayoferythrocytecholesterol AT jleggitt measurementofinvivocholesterolsynthesisfrom2h2oarapidprocedurefortheisolationcombustionandisotopicassayoferythrocytecholesterol AT llclarke measurementofinvivocholesterolsynthesisfrom2h2oarapidprocedurefortheisolationcombustionandisotopicassayoferythrocytecholesterol AT wgpond measurementofinvivocholesterolsynthesisfrom2h2oarapidprocedurefortheisolationcombustionandisotopicassayoferythrocytecholesterol AT pdklein measurementofinvivocholesterolsynthesisfrom2h2oarapidprocedurefortheisolationcombustionandisotopicassayoferythrocytecholesterol |