DNA-free high-quality RNA extraction from 39 difficult-to-extract plant species (representing seasonal tissues and tissue types) of 32 families, and its validation for downstream molecular applications

Abstract Background High-purity RNA serves as the basic requirement for downstream molecular analysis of plant species, especially the differential expression of genes to various biotic and abiotic stimuli. But, the extraction of high-quality RNA is usually difficult from plants rich in polysacchari...

Full description

Bibliographic Details
Main Authors: Shina Sasi, Saranya Krishnan, Preshobha Kodackattumannil, Aysha AL Shamisi, Maitha Aldarmaki, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Format: Article
Language:English
Published: BMC 2023-08-01
Series:Plant Methods
Subjects:
Online Access:https://doi.org/10.1186/s13007-023-01063-5