DNA-free high-quality RNA extraction from 39 difficult-to-extract plant species (representing seasonal tissues and tissue types) of 32 families, and its validation for downstream molecular applications
Abstract Background High-purity RNA serves as the basic requirement for downstream molecular analysis of plant species, especially the differential expression of genes to various biotic and abiotic stimuli. But, the extraction of high-quality RNA is usually difficult from plants rich in polysacchari...
Main Authors: | , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2023-08-01
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Series: | Plant Methods |
Subjects: | |
Online Access: | https://doi.org/10.1186/s13007-023-01063-5 |