Regulation Mechanisms of Meiotic Recombination Revealed from the Analysis of a Fission Yeast Recombination Hotspot <i>ade6-M26</i>
Meiotic recombination is a pivotal event that ensures faithful chromosome segregation and creates genetic diversity in gametes. Meiotic recombination is initiated by programmed double-strand breaks (DSBs), which are catalyzed by the conserved Spo11 protein. Spo11 is an enzyme with structural similar...
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MDPI AG
2022-11-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/12/1761 |
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| author | Kouji Hirota |
| author_facet | Kouji Hirota |
| author_sort | Kouji Hirota |
| collection | DOAJ |
| description | Meiotic recombination is a pivotal event that ensures faithful chromosome segregation and creates genetic diversity in gametes. Meiotic recombination is initiated by programmed double-strand breaks (DSBs), which are catalyzed by the conserved Spo11 protein. Spo11 is an enzyme with structural similarity to topoisomerase II and induces DSBs through the nucleophilic attack of the phosphodiester bond by the hydroxy group of its tyrosine (Tyr) catalytic residue. DSBs caused by Spo11 are repaired by homologous recombination using homologous chromosomes as donors, resulting in crossovers/chiasmata, which ensure physical contact between homologous chromosomes. Thus, the site of meiotic recombination is determined by the site of the induced DSB on the chromosome. Meiotic recombination is not uniformly induced, and sites showing high recombination rates are referred to as recombination hotspots. In fission yeast, <i>ade6-M26</i>, a nonsense point mutation of <i>ade6</i> is a well-characterized meiotic recombination hotspot caused by the heptanucleotide sequence 5′-ATGACGT-3′ at the <i>M26</i> mutation point. In this review, we summarize the meiotic recombination mechanisms revealed by the analysis of the fission <i>ade6-M26</i> gene as a model system. |
| first_indexed | 2024-03-09T17:17:39Z |
| format | Article |
| id | doaj.art-ce85cd8a6124434087cd68a6fc951a97 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T17:17:39Z |
| publishDate | 2022-11-01 |
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| series | Biomolecules |
| spelling | doaj.art-ce85cd8a6124434087cd68a6fc951a972023-11-24T13:32:52ZengMDPI AGBiomolecules2218-273X2022-11-011212176110.3390/biom12121761Regulation Mechanisms of Meiotic Recombination Revealed from the Analysis of a Fission Yeast Recombination Hotspot <i>ade6-M26</i>Kouji Hirota0Department of Chemistry, Graduate School of Science, Tokyo Metropolitan University, Minamiosawa 1-1, Hachioji-shi, Tokyo 192-0397, JapanMeiotic recombination is a pivotal event that ensures faithful chromosome segregation and creates genetic diversity in gametes. Meiotic recombination is initiated by programmed double-strand breaks (DSBs), which are catalyzed by the conserved Spo11 protein. Spo11 is an enzyme with structural similarity to topoisomerase II and induces DSBs through the nucleophilic attack of the phosphodiester bond by the hydroxy group of its tyrosine (Tyr) catalytic residue. DSBs caused by Spo11 are repaired by homologous recombination using homologous chromosomes as donors, resulting in crossovers/chiasmata, which ensure physical contact between homologous chromosomes. Thus, the site of meiotic recombination is determined by the site of the induced DSB on the chromosome. Meiotic recombination is not uniformly induced, and sites showing high recombination rates are referred to as recombination hotspots. In fission yeast, <i>ade6-M26</i>, a nonsense point mutation of <i>ade6</i> is a well-characterized meiotic recombination hotspot caused by the heptanucleotide sequence 5′-ATGACGT-3′ at the <i>M26</i> mutation point. In this review, we summarize the meiotic recombination mechanisms revealed by the analysis of the fission <i>ade6-M26</i> gene as a model system.https://www.mdpi.com/2218-273X/12/12/1761meiotic recombinationdouble strand break (DSB)Rec12 (fission yeast Spo11 homolog)chromatintranscription factor<i>S. pombe</i> |
| spellingShingle | Kouji Hirota Regulation Mechanisms of Meiotic Recombination Revealed from the Analysis of a Fission Yeast Recombination Hotspot <i>ade6-M26</i> Biomolecules meiotic recombination double strand break (DSB) Rec12 (fission yeast Spo11 homolog) chromatin transcription factor <i>S. pombe</i> |
| title | Regulation Mechanisms of Meiotic Recombination Revealed from the Analysis of a Fission Yeast Recombination Hotspot <i>ade6-M26</i> |
| title_full | Regulation Mechanisms of Meiotic Recombination Revealed from the Analysis of a Fission Yeast Recombination Hotspot <i>ade6-M26</i> |
| title_fullStr | Regulation Mechanisms of Meiotic Recombination Revealed from the Analysis of a Fission Yeast Recombination Hotspot <i>ade6-M26</i> |
| title_full_unstemmed | Regulation Mechanisms of Meiotic Recombination Revealed from the Analysis of a Fission Yeast Recombination Hotspot <i>ade6-M26</i> |
| title_short | Regulation Mechanisms of Meiotic Recombination Revealed from the Analysis of a Fission Yeast Recombination Hotspot <i>ade6-M26</i> |
| title_sort | regulation mechanisms of meiotic recombination revealed from the analysis of a fission yeast recombination hotspot i ade6 m26 i |
| topic | meiotic recombination double strand break (DSB) Rec12 (fission yeast Spo11 homolog) chromatin transcription factor <i>S. pombe</i> |
| url | https://www.mdpi.com/2218-273X/12/12/1761 |
| work_keys_str_mv | AT koujihirota regulationmechanismsofmeioticrecombinationrevealedfromtheanalysisofafissionyeastrecombinationhotspotiade6m26i |