Protocol for constructing GQD-PMO functionalized FET-biosensor for ultrasensitive exosomal miRNA detection

Summary: Here, we present a protocol for constructing an ultrasensitive biosensor for exosomal-miRNA detection. We describe steps for preparing graphene quantum dot-phosphorodiamidate morpholino oligomer hybrids, depositing them onto the reduced graphene oxide field surface, hybridizing analyte miRN...

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Bibliographic Details
Main Authors: Kun Li, Chunzi Liang, Yuling Pan, Linghan Mei, Guo-Jun Zhang
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723004835