Protocol for constructing GQD-PMO functionalized FET-biosensor for ultrasensitive exosomal miRNA detection
Summary: Here, we present a protocol for constructing an ultrasensitive biosensor for exosomal-miRNA detection. We describe steps for preparing graphene quantum dot-phosphorodiamidate morpholino oligomer hybrids, depositing them onto the reduced graphene oxide field surface, hybridizing analyte miRN...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-09-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723004835 |
| _version_ | 1828717394030231552 |
|---|---|
| author | Kun Li Chunzi Liang Yuling Pan Linghan Mei Guo-Jun Zhang |
| author_facet | Kun Li Chunzi Liang Yuling Pan Linghan Mei Guo-Jun Zhang |
| author_sort | Kun Li |
| collection | DOAJ |
| description | Summary: Here, we present a protocol for constructing an ultrasensitive biosensor for exosomal-miRNA detection. We describe steps for preparing graphene quantum dot-phosphorodiamidate morpholino oligomer hybrids, depositing them onto the reduced graphene oxide field surface, hybridizing analyte miRNA with the sensor probe, and capturing and calculating electrical signals. We also detail procedures for optimizing biosensor construction and evaluating performance. By quantifying plasma exosomal miRNA21, this protocol can identify cancer patients from healthy individuals.For complete details on the use and execution of this protocol, please refer to Li et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-12T14:26:42Z |
| format | Article |
| id | doaj.art-ce86b5e915ee4a28a254a551a015bce9 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-12T14:26:42Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-ce86b5e915ee4a28a254a551a015bce92023-08-18T04:31:35ZengElsevierSTAR Protocols2666-16672023-09-0143102516Protocol for constructing GQD-PMO functionalized FET-biosensor for ultrasensitive exosomal miRNA detectionKun Li0Chunzi Liang1Yuling Pan2Linghan Mei3Guo-Jun Zhang4Hubei University of Chinese Medicine, Wuhan City, Hubei Province 430073, China; Department of Medical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei University of Chinese Medicine, Wuhan City, Hubei Province 430073, China; Corresponding authorHubei University of Chinese Medicine, Wuhan City, Hubei Province 430073, ChinaHubei University of Chinese Medicine, Wuhan City, Hubei Province 430073, ChinaHubei University of Chinese Medicine, Wuhan City, Hubei Province 430073, China; Corresponding authorSummary: Here, we present a protocol for constructing an ultrasensitive biosensor for exosomal-miRNA detection. We describe steps for preparing graphene quantum dot-phosphorodiamidate morpholino oligomer hybrids, depositing them onto the reduced graphene oxide field surface, hybridizing analyte miRNA with the sensor probe, and capturing and calculating electrical signals. We also detail procedures for optimizing biosensor construction and evaluating performance. By quantifying plasma exosomal miRNA21, this protocol can identify cancer patients from healthy individuals.For complete details on the use and execution of this protocol, please refer to Li et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723004835CancerBiotechnology and BioengineeringChemistry |
| spellingShingle | Kun Li Chunzi Liang Yuling Pan Linghan Mei Guo-Jun Zhang Protocol for constructing GQD-PMO functionalized FET-biosensor for ultrasensitive exosomal miRNA detection STAR Protocols Cancer Biotechnology and Bioengineering Chemistry |
| title | Protocol for constructing GQD-PMO functionalized FET-biosensor for ultrasensitive exosomal miRNA detection |
| title_full | Protocol for constructing GQD-PMO functionalized FET-biosensor for ultrasensitive exosomal miRNA detection |
| title_fullStr | Protocol for constructing GQD-PMO functionalized FET-biosensor for ultrasensitive exosomal miRNA detection |
| title_full_unstemmed | Protocol for constructing GQD-PMO functionalized FET-biosensor for ultrasensitive exosomal miRNA detection |
| title_short | Protocol for constructing GQD-PMO functionalized FET-biosensor for ultrasensitive exosomal miRNA detection |
| title_sort | protocol for constructing gqd pmo functionalized fet biosensor for ultrasensitive exosomal mirna detection |
| topic | Cancer Biotechnology and Bioengineering Chemistry |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723004835 |
| work_keys_str_mv | AT kunli protocolforconstructinggqdpmofunctionalizedfetbiosensorforultrasensitiveexosomalmirnadetection AT chunziliang protocolforconstructinggqdpmofunctionalizedfetbiosensorforultrasensitiveexosomalmirnadetection AT yulingpan protocolforconstructinggqdpmofunctionalizedfetbiosensorforultrasensitiveexosomalmirnadetection AT linghanmei protocolforconstructinggqdpmofunctionalizedfetbiosensorforultrasensitiveexosomalmirnadetection AT guojunzhang protocolforconstructinggqdpmofunctionalizedfetbiosensorforultrasensitiveexosomalmirnadetection |