MarShie: a clearing protocol for 3D analysis of single cells throughout the bone marrow at subcellular resolution

Abstract Analyzing immune cell interactions in the bone marrow is vital for understanding hematopoiesis and bone homeostasis. Three-dimensional analysis of the complete, intact bone marrow within the cortex of whole long bones remains a challenge, especially at subcellular resolution. We present a m...

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Bibliographic Details
Main Authors: Till Fabian Mertens, Alina Tabea Liebheit, Johanna Ehl, Ralf Köhler, Asylkhan Rakhymzhan, Andrew Woehler, Lukas Katthän, Gernot Ebel, Wjatscheslaw Liublin, Ana Kasapi, Antigoni Triantafyllopoulou, Tim Julius Schulz, Raluca Aura Niesner, Anja Erika Hauser
Format: Article
Language:English
Published: Nature Portfolio 2024-02-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-024-45827-6