Precisely Monomeric Linear RNAs of Viroids Belonging to <i>Pospiviroid</i> and <i>Hostuviroid</i> Genera Are Infectious Regardless of Transcription Initiation Site and 5′-Terminal Structure
Infectious dimeric RNA transcripts are a powerful tool for reverse genetic analyses in viroid studies. However, the construction of dimeric cDNA clones is laborious and time consuming, especially in mutational analyses by in vitro mutagenesis. In this study, we developed a system to synthesize a pre...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
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| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/10/11/2971 |