The effects of freezing on faecal microbiota as determined using MiSeq sequencing and culture-based investigations.

High-throughput sequencing has enabled detailed insights into complex microbial environments, including the human gut microbiota. The accuracy of the sequencing data however, is reliant upon appropriate storage of the samples prior to DNA extraction. The aim of this study was to conduct the first Mi...

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Bibliographic Details
Main Authors: Fiona Fouhy, Jennifer Deane, Mary C Rea, Órla O'Sullivan, R Paul Ross, Grace O'Callaghan, Barry J Plant, Catherine Stanton
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4352061?pdf=render