Iron accumulation and partitioning in hydroponically grown wild and cultivated chickpea (Cicer arietinum L)
Chickpea (Cicer arietinum L.) is a staple food in many developing countries where iron (Fe) deficiency often occurs in their population. The crop is a good source of protein, vitamins, and micronutrients. Fe biofortification in chickpea can be part of long-term strategy to enhance Fe intake in human...
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| Format: | Article |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1092493/full |
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| author | Tamanna A. Jahan Shweta Kalve Zachery Belak Christopher Eskiw Bunyamin Tar’an |
| author_facet | Tamanna A. Jahan Shweta Kalve Zachery Belak Christopher Eskiw Bunyamin Tar’an |
| author_sort | Tamanna A. Jahan |
| collection | DOAJ |
| description | Chickpea (Cicer arietinum L.) is a staple food in many developing countries where iron (Fe) deficiency often occurs in their population. The crop is a good source of protein, vitamins, and micronutrients. Fe biofortification in chickpea can be part of long-term strategy to enhance Fe intake in human diet to help to alleviate Fe deficiency. To develop cultivars with high Fe concentration in seeds, understanding the mechanisms of absorption and translocation of Fe into the seeds is critical. An experiment was conducted using a hydroponic system to evaluate Fe accumulation in seeds and other organs at different growth stages of selected genotypes of cultivated and wild relatives of chickpea. Plants were grown in media with Fe zero and Fe added conditions. Six chickpea genotypes were grown and harvested at six different growth stages: V3, V10, R2, R5, R6, and RH for analysis of Fe concentration in roots, stems, leaves, and seeds. The relative expression of genes related to Fe-metabolism including FRO2, IRT1, NRAMP3, V1T1, YSL1, FER3, GCN2, and WEE1 was analyzed. The results showed that the highest and lowest accumulation of Fe throughout the plant growth stages were found in the roots and stems, respectively. Results of gene expression analysis confirmed that the FRO2 and IRT1 were involved in Fe uptake in chickpeas and expressed more in roots under Fe added condition. All transporter genes: NRAMP3, V1T1, YSL1 along with storage gene FER3 showed higher expression in leaves. In contrast, candidate gene WEE1 for Fe metabolism expressed more in roots under Fe affluent condition; however, GCN2 showed over-expression in roots under Fe zero condition. Current finding will contribute to better understanding of Fe translocation and metabolism in chickpea. This knowledge can further be used to develop chickpea varieties with high Fe in seeds. |
| first_indexed | 2024-04-09T23:54:01Z |
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| id | doaj.art-cebc82b1f2e74b1cbac02dada3f1f89f |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-09T23:54:01Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-cebc82b1f2e74b1cbac02dada3f1f89f2023-03-17T04:48:55ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-03-011410.3389/fpls.2023.10924931092493Iron accumulation and partitioning in hydroponically grown wild and cultivated chickpea (Cicer arietinum L)Tamanna A. Jahan0Shweta Kalve1Zachery Belak2Christopher Eskiw3Bunyamin Tar’an4Department of Plant Sciences, College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK, CanadaDepartment of Plant Sciences, College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK, CanadaDepartment of Food and Bioproduct Sciences, College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK, CanadaDepartment of Food and Bioproduct Sciences, College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK, CanadaDepartment of Plant Sciences, College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK, CanadaChickpea (Cicer arietinum L.) is a staple food in many developing countries where iron (Fe) deficiency often occurs in their population. The crop is a good source of protein, vitamins, and micronutrients. Fe biofortification in chickpea can be part of long-term strategy to enhance Fe intake in human diet to help to alleviate Fe deficiency. To develop cultivars with high Fe concentration in seeds, understanding the mechanisms of absorption and translocation of Fe into the seeds is critical. An experiment was conducted using a hydroponic system to evaluate Fe accumulation in seeds and other organs at different growth stages of selected genotypes of cultivated and wild relatives of chickpea. Plants were grown in media with Fe zero and Fe added conditions. Six chickpea genotypes were grown and harvested at six different growth stages: V3, V10, R2, R5, R6, and RH for analysis of Fe concentration in roots, stems, leaves, and seeds. The relative expression of genes related to Fe-metabolism including FRO2, IRT1, NRAMP3, V1T1, YSL1, FER3, GCN2, and WEE1 was analyzed. The results showed that the highest and lowest accumulation of Fe throughout the plant growth stages were found in the roots and stems, respectively. Results of gene expression analysis confirmed that the FRO2 and IRT1 were involved in Fe uptake in chickpeas and expressed more in roots under Fe added condition. All transporter genes: NRAMP3, V1T1, YSL1 along with storage gene FER3 showed higher expression in leaves. In contrast, candidate gene WEE1 for Fe metabolism expressed more in roots under Fe affluent condition; however, GCN2 showed over-expression in roots under Fe zero condition. Current finding will contribute to better understanding of Fe translocation and metabolism in chickpea. This knowledge can further be used to develop chickpea varieties with high Fe in seeds.https://www.frontiersin.org/articles/10.3389/fpls.2023.1092493/fullFe accumulationFe translocationgene expressionchickpeabiofortification |
| spellingShingle | Tamanna A. Jahan Shweta Kalve Zachery Belak Christopher Eskiw Bunyamin Tar’an Iron accumulation and partitioning in hydroponically grown wild and cultivated chickpea (Cicer arietinum L) Frontiers in Plant Science Fe accumulation Fe translocation gene expression chickpea biofortification |
| title | Iron accumulation and partitioning in hydroponically grown wild and cultivated chickpea (Cicer arietinum L) |
| title_full | Iron accumulation and partitioning in hydroponically grown wild and cultivated chickpea (Cicer arietinum L) |
| title_fullStr | Iron accumulation and partitioning in hydroponically grown wild and cultivated chickpea (Cicer arietinum L) |
| title_full_unstemmed | Iron accumulation and partitioning in hydroponically grown wild and cultivated chickpea (Cicer arietinum L) |
| title_short | Iron accumulation and partitioning in hydroponically grown wild and cultivated chickpea (Cicer arietinum L) |
| title_sort | iron accumulation and partitioning in hydroponically grown wild and cultivated chickpea cicer arietinum l |
| topic | Fe accumulation Fe translocation gene expression chickpea biofortification |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1092493/full |
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