Oral ingestion of Shiikuwasha extract suppresses diabetes progression in db/db mice by preserving β-cell mass
IntroductionNobiletin is a polymethoxyflavonoid abundant in citrus peels and has been reported to have various bioactive effects. We have previously reported that nobiletin inhibits endoplasmic reticulum stress-induced apoptosis in the pancreatic β-cell line INS-1 and that continuous subcutaneous ad...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2023.1336133/full |
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| author | Megumi Kaji Yukiko K. Kaneko Stella Amarachi Ihim Ran Kanoh Moe Yamamoto Momoka Yamaguchi Tomohisa Ishikawa |
| author_facet | Megumi Kaji Yukiko K. Kaneko Stella Amarachi Ihim Ran Kanoh Moe Yamamoto Momoka Yamaguchi Tomohisa Ishikawa |
| author_sort | Megumi Kaji |
| collection | DOAJ |
| description | IntroductionNobiletin is a polymethoxyflavonoid abundant in citrus peels and has been reported to have various bioactive effects. We have previously reported that nobiletin inhibits endoplasmic reticulum stress-induced apoptosis in the pancreatic β-cell line INS-1 and that continuous subcutaneous administration of nobiletin suppresses the progression of diabetes by protecting β-cells in type 2 diabetic db/db mice. In the present study, we investigated effects of oral ingestion of Shiikuwasha extract rich in nobiletin on the pathogenesis of type 2 diabetes in db/db mice.Materials and methodsA Shiikuwasha extract was dissolved in MediDrop sucralose. Twenty-four mice were equally divided in three groups and fed with vehicle or low or high dose of Shiikuwasha extract for 4 weeks. Blood glucose levels, pancreatic β-cell mass, serum insulin levels, pancreatic insulin content, and other biomarkers were measured and compared between the groups.ResultsThe group that freely ingested the Shiikuwasha extract containing higher concentration of nobiletin (Shiikuwasha H) showed lower blood glucose levels. At the end of the experiment, the Shiikuwasha H group exhibited improved glucose tolerance, lower serum glycoalbumin levels, and an increase in β-cell area per pancreas compared with the control group. Body weight, food intake, and serum biomarkers related to liver function and lipid metabolism of the Shiikuwasha H group were not different from those of the control group, although water intake of the former was significantly decreased than that of the latter.ConclusionOur results suggest that the oral ingestion of Shiikuwasha extract preserves pancreatic β-cell mass in diabetic mice, which might be attributed to ameliorating the progression of diabetes. |
| first_indexed | 2024-03-08T16:50:26Z |
| format | Article |
| id | doaj.art-cefd470eca1545a4bf29a0362286918a |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-03-08T16:50:26Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-cefd470eca1545a4bf29a0362286918a2024-01-05T05:15:05ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2024-01-011010.3389/fnut.2023.13361331336133Oral ingestion of Shiikuwasha extract suppresses diabetes progression in db/db mice by preserving β-cell massMegumi KajiYukiko K. KanekoStella Amarachi IhimRan KanohMoe YamamotoMomoka YamaguchiTomohisa IshikawaIntroductionNobiletin is a polymethoxyflavonoid abundant in citrus peels and has been reported to have various bioactive effects. We have previously reported that nobiletin inhibits endoplasmic reticulum stress-induced apoptosis in the pancreatic β-cell line INS-1 and that continuous subcutaneous administration of nobiletin suppresses the progression of diabetes by protecting β-cells in type 2 diabetic db/db mice. In the present study, we investigated effects of oral ingestion of Shiikuwasha extract rich in nobiletin on the pathogenesis of type 2 diabetes in db/db mice.Materials and methodsA Shiikuwasha extract was dissolved in MediDrop sucralose. Twenty-four mice were equally divided in three groups and fed with vehicle or low or high dose of Shiikuwasha extract for 4 weeks. Blood glucose levels, pancreatic β-cell mass, serum insulin levels, pancreatic insulin content, and other biomarkers were measured and compared between the groups.ResultsThe group that freely ingested the Shiikuwasha extract containing higher concentration of nobiletin (Shiikuwasha H) showed lower blood glucose levels. At the end of the experiment, the Shiikuwasha H group exhibited improved glucose tolerance, lower serum glycoalbumin levels, and an increase in β-cell area per pancreas compared with the control group. Body weight, food intake, and serum biomarkers related to liver function and lipid metabolism of the Shiikuwasha H group were not different from those of the control group, although water intake of the former was significantly decreased than that of the latter.ConclusionOur results suggest that the oral ingestion of Shiikuwasha extract preserves pancreatic β-cell mass in diabetic mice, which might be attributed to ameliorating the progression of diabetes.https://www.frontiersin.org/articles/10.3389/fnut.2023.1336133/fullShiikuwasha extractnobiletintype 2 diabetesdb/db micepancreatic β-cellsinsulin |
| spellingShingle | Megumi Kaji Yukiko K. Kaneko Stella Amarachi Ihim Ran Kanoh Moe Yamamoto Momoka Yamaguchi Tomohisa Ishikawa Oral ingestion of Shiikuwasha extract suppresses diabetes progression in db/db mice by preserving β-cell mass Frontiers in Nutrition Shiikuwasha extract nobiletin type 2 diabetes db/db mice pancreatic β-cells insulin |
| title | Oral ingestion of Shiikuwasha extract suppresses diabetes progression in db/db mice by preserving β-cell mass |
| title_full | Oral ingestion of Shiikuwasha extract suppresses diabetes progression in db/db mice by preserving β-cell mass |
| title_fullStr | Oral ingestion of Shiikuwasha extract suppresses diabetes progression in db/db mice by preserving β-cell mass |
| title_full_unstemmed | Oral ingestion of Shiikuwasha extract suppresses diabetes progression in db/db mice by preserving β-cell mass |
| title_short | Oral ingestion of Shiikuwasha extract suppresses diabetes progression in db/db mice by preserving β-cell mass |
| title_sort | oral ingestion of shiikuwasha extract suppresses diabetes progression in db db mice by preserving β cell mass |
| topic | Shiikuwasha extract nobiletin type 2 diabetes db/db mice pancreatic β-cells insulin |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2023.1336133/full |
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