Screening of Locally Isolated Lactic Acid Bacteria for Use as Probiotics in Poultry in Iran
A total of 250 chicken intestinal specimens were screened for the presence of lactic acid bacteria (LAB) by routine cultural, morphological and biochemical reactions. Based on the results, 75 isolates were identified as LAB and were characterized for their probiotic potential. During preliminary i...
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2007-10-01
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| Series: | The Journal of Poultry Science |
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| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/44/4/44_4_357/_pdf/-char/en |
| _version_ | 1797816282885652480 |
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| author | Naheed Mojgani Mohd A. K. Torshizi Shaban Rahimi |
| author_facet | Naheed Mojgani Mohd A. K. Torshizi Shaban Rahimi |
| author_sort | Naheed Mojgani |
| collection | DOAJ |
| description | A total of 250 chicken intestinal specimens were screened for the presence of lactic acid bacteria (LAB) by routine cultural, morphological and biochemical reactions. Based on the results, 75 isolates were identified as LAB and were characterized for their probiotic potential. During preliminary investigations, the viability of the isolated LAB strains under different environmental and stress conditions was evaluated. Three of the strains which appeared resistant to acidic pH values and high bile salt concentrations were selected for further detailed studies. Based on the biochemical reactions these strains were identified as, Lactobacillus acidophilus RNL26, Lactobacillus fermentum RNL 44, and Lactobacillus salivarius RNL 49. All three strains demonstrated enhanced inhibitory activity against gram positive species including Clostridium perfringens, Staphylococcus aureus and Listeria monocytogene, while L. fermentum RNL 44 was also inhibitory towards gram negative bacteria; Salmonella enteritidis and S. typhimurium. The antibacterial effect exerted by L. acidophilus RNL26 and L. salivarius RNL 49 appeared to be due to bacteriocin or bacteriocin like substance, as the neutralized and catalase treated supernatant fluid of both the strains retained their antibacterial activity. All three strains were further characterized for their cholesterol removal, aggregation, co-aggregation, and cell surface hydrophobicity under in vitro conditions. According to our results L. fermentum RNL 44 appeared to be a superior probiotic candidate compared to the other two studied probiotic strains, Lactobacillus acidophilus RNL26 and Lactobacillus salivarius RNL 49. |
| first_indexed | 2024-03-13T08:35:26Z |
| format | Article |
| id | doaj.art-cf07a4163cba4c868b4ab599ac3c8adc |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:35:26Z |
| publishDate | 2007-10-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-cf07a4163cba4c868b4ab599ac3c8adc2023-05-31T00:27:52ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862007-10-0144435736510.2141/jpsa.44.357jpsaScreening of Locally Isolated Lactic Acid Bacteria for Use as Probiotics in Poultry in IranNaheed Mojgani0Mohd A. K. Torshizi1Shaban Rahimi2Department of Biotechnology, Razi Vaccine and Serum Research Institute, KarajDepartment of Animal Science, College of Agriculture, Tarbiat Modarres University, IranDepartment of Biotechnology, Razi Vaccine and Serum Research Institute, KarajA total of 250 chicken intestinal specimens were screened for the presence of lactic acid bacteria (LAB) by routine cultural, morphological and biochemical reactions. Based on the results, 75 isolates were identified as LAB and were characterized for their probiotic potential. During preliminary investigations, the viability of the isolated LAB strains under different environmental and stress conditions was evaluated. Three of the strains which appeared resistant to acidic pH values and high bile salt concentrations were selected for further detailed studies. Based on the biochemical reactions these strains were identified as, Lactobacillus acidophilus RNL26, Lactobacillus fermentum RNL 44, and Lactobacillus salivarius RNL 49. All three strains demonstrated enhanced inhibitory activity against gram positive species including Clostridium perfringens, Staphylococcus aureus and Listeria monocytogene, while L. fermentum RNL 44 was also inhibitory towards gram negative bacteria; Salmonella enteritidis and S. typhimurium. The antibacterial effect exerted by L. acidophilus RNL26 and L. salivarius RNL 49 appeared to be due to bacteriocin or bacteriocin like substance, as the neutralized and catalase treated supernatant fluid of both the strains retained their antibacterial activity. All three strains were further characterized for their cholesterol removal, aggregation, co-aggregation, and cell surface hydrophobicity under in vitro conditions. According to our results L. fermentum RNL 44 appeared to be a superior probiotic candidate compared to the other two studied probiotic strains, Lactobacillus acidophilus RNL26 and Lactobacillus salivarius RNL 49.https://www.jstage.jst.go.jp/article/jpsa/44/4/44_4_357/_pdf/-char/enantimicrobialin vitrolactic acid bacteriaprobiotic potential |
| spellingShingle | Naheed Mojgani Mohd A. K. Torshizi Shaban Rahimi Screening of Locally Isolated Lactic Acid Bacteria for Use as Probiotics in Poultry in Iran The Journal of Poultry Science antimicrobial in vitro lactic acid bacteria probiotic potential |
| title | Screening of Locally Isolated Lactic Acid Bacteria for Use as Probiotics in Poultry in Iran |
| title_full | Screening of Locally Isolated Lactic Acid Bacteria for Use as Probiotics in Poultry in Iran |
| title_fullStr | Screening of Locally Isolated Lactic Acid Bacteria for Use as Probiotics in Poultry in Iran |
| title_full_unstemmed | Screening of Locally Isolated Lactic Acid Bacteria for Use as Probiotics in Poultry in Iran |
| title_short | Screening of Locally Isolated Lactic Acid Bacteria for Use as Probiotics in Poultry in Iran |
| title_sort | screening of locally isolated lactic acid bacteria for use as probiotics in poultry in iran |
| topic | antimicrobial in vitro lactic acid bacteria probiotic potential |
| url | https://www.jstage.jst.go.jp/article/jpsa/44/4/44_4_357/_pdf/-char/en |
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