Recombinant Expression and Allergenicity Identification of Sesame Allergen Ses i 3
Total RNA was extracted from sesame seeds, and the sesame Ses i 3 gene was obtained by using reverse transcription-polymerase chain reaction (RT-PCR). The plasmid pET-22b(+) was used to construct an expression vector, and the recombinant plasmid was transformed into competent Escherichia coli BL21 (...
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Format: | Article |
Language: | English |
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China Food Publishing Company
2023-11-01
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Series: | Shipin Kexue |
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Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-22-016.pdf |