Recombinant Expression and Allergenicity Identification of Sesame Allergen Ses i 3
Total RNA was extracted from sesame seeds, and the sesame Ses i 3 gene was obtained by using reverse transcription-polymerase chain reaction (RT-PCR). The plasmid pET-22b(+) was used to construct an expression vector, and the recombinant plasmid was transformed into competent Escherichia coli BL21 (...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2023-11-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-22-016.pdf |
| _version_ | 1797376166524354560 |
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| author | JIANG Songsong, WANG Tao, TANG Xinlei, LI Qian, HUANG Yutong, YU Dandan, MENG Xiangren |
| author_facet | JIANG Songsong, WANG Tao, TANG Xinlei, LI Qian, HUANG Yutong, YU Dandan, MENG Xiangren |
| author_sort | JIANG Songsong, WANG Tao, TANG Xinlei, LI Qian, HUANG Yutong, YU Dandan, MENG Xiangren |
| collection | DOAJ |
| description | Total RNA was extracted from sesame seeds, and the sesame Ses i 3 gene was obtained by using reverse transcription-polymerase chain reaction (RT-PCR). The plasmid pET-22b(+) was used to construct an expression vector, and the recombinant plasmid was transformed into competent Escherichia coli BL21 (DE3) host cells for induced expression. The expressed protein was purified by Ni2+-affinity chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that the molecular mass of the expressed protein was approximately 66 kDa. The ability of the recombinant and native Ses i 3 protein to induce sesame allergy in mice was compared by using a BALB/c mouse allergy model, which was found to be similar by measuring specific antibodies (immunoglobulin E (IgE), IgG1, and IgG2a), cytokines (interleukin (IL)-4, IL-5, interferon-γ (IFN-γ) and histamines). Hence, the recombinant Ses i 3 protein can be used for future studies on the allergenicity of sesame. |
| first_indexed | 2024-03-08T19:34:46Z |
| format | Article |
| id | doaj.art-cf205199e20a4380868bd1747e13bc91 |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-03-08T19:34:46Z |
| publishDate | 2023-11-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-cf205199e20a4380868bd1747e13bc912023-12-26T06:45:18ZengChina Food Publishing CompanyShipin Kexue1002-66302023-11-01442212613210.7506/spkx1002-6630-20230212-109Recombinant Expression and Allergenicity Identification of Sesame Allergen Ses i 3JIANG Songsong, WANG Tao, TANG Xinlei, LI Qian, HUANG Yutong, YU Dandan, MENG Xiangren0(1. College of Tourism and Culinary, Yangzhou University, Yangzhou 225127, China;2. Chinese Cuisine Promotion and Research Base, Yangzhou University, Yangzhou 225127, China)Total RNA was extracted from sesame seeds, and the sesame Ses i 3 gene was obtained by using reverse transcription-polymerase chain reaction (RT-PCR). The plasmid pET-22b(+) was used to construct an expression vector, and the recombinant plasmid was transformed into competent Escherichia coli BL21 (DE3) host cells for induced expression. The expressed protein was purified by Ni2+-affinity chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that the molecular mass of the expressed protein was approximately 66 kDa. The ability of the recombinant and native Ses i 3 protein to induce sesame allergy in mice was compared by using a BALB/c mouse allergy model, which was found to be similar by measuring specific antibodies (immunoglobulin E (IgE), IgG1, and IgG2a), cytokines (interleukin (IL)-4, IL-5, interferon-γ (IFN-γ) and histamines). Hence, the recombinant Ses i 3 protein can be used for future studies on the allergenicity of sesame.https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-22-016.pdfsesame allergen; ses i 3; recombinant protein; allergenicity; balb/c mice |
| spellingShingle | JIANG Songsong, WANG Tao, TANG Xinlei, LI Qian, HUANG Yutong, YU Dandan, MENG Xiangren Recombinant Expression and Allergenicity Identification of Sesame Allergen Ses i 3 Shipin Kexue sesame allergen; ses i 3; recombinant protein; allergenicity; balb/c mice |
| title | Recombinant Expression and Allergenicity Identification of Sesame Allergen Ses i 3 |
| title_full | Recombinant Expression and Allergenicity Identification of Sesame Allergen Ses i 3 |
| title_fullStr | Recombinant Expression and Allergenicity Identification of Sesame Allergen Ses i 3 |
| title_full_unstemmed | Recombinant Expression and Allergenicity Identification of Sesame Allergen Ses i 3 |
| title_short | Recombinant Expression and Allergenicity Identification of Sesame Allergen Ses i 3 |
| title_sort | recombinant expression and allergenicity identification of sesame allergen ses i 3 |
| topic | sesame allergen; ses i 3; recombinant protein; allergenicity; balb/c mice |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-22-016.pdf |
| work_keys_str_mv | AT jiangsongsongwangtaotangxinleiliqianhuangyutongyudandanmengxiangren recombinantexpressionandallergenicityidentificationofsesameallergensesi3 |