Use of anionic denaturing detergents to purify insoluble proteins after overexpression
<p>Background</p> <p>Many proteins form insoluble protein aggregates, called “inclusion bodies”, when overexpressed in <it>E. coli</it>. This is the biggest obstacle in biotechnology. Ever since the reversible denaturation of proteins by chaotropic agents such as urea o...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-12-01
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| Series: | BMC Biotechnology |
| Subjects: | |
| Online Access: | http://www.biomedcentral.com/1472-6750/12/95 |