A new technical approach for preparing frozen biological samples for electron microscopy

A new technical approach for preparing frozen biological samples for electron microscopy

Abstract Background Many methodological approaches have focused so far on physiological and molecular responses of plant tissues to freezing but only little knowledge is available on the consequences of extracellular ice-formation on cellular ultrastructure that underlies physiological reactions. In...

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Bibliographic Details
Main Authors: Othmar Buchner, Philip Steiner, Ancuela Andosch, Andreas Holzinger, Matthias Stegner, Gilbert Neuner, Ursula Lütz-Meindl
Format: Article
Language:English
Published: BMC 2020-04-01
Series:Plant Methods
Subjects:
Cell organelles
High pressure freeze fixation
Klebsormidium crenulatum
Lemna sp.
Micrasterias denticulata
Pinus mugo
Online Access:http://link.springer.com/article/10.1186/s13007-020-00586-5

Internet

http://link.springer.com/article/10.1186/s13007-020-00586-5

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