3D time-lapse microscopy paired with endpoint lineage analysis in mouse blastocysts

Summary: Determining how signaling dynamics relate to gene expression and cell fate is essential to understanding multicellular development. We present a unified live imaging and lineage analysis method that allows integrated analysis of both techniques in the same mouse embryos. This protocol descr...

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Bibliographic Details
Main Authors: Michael J. Pokrass, Sergi Regot
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721001532