Combining microfluidics, optogenetics and calcium imaging to study neuronal communication in vitro.

Combining microfluidics, optogenetics and calcium imaging to study neuronal communication in vitro.

In this paper we report the combination of microfluidics, optogenetics and calcium imaging as a cheap and convenient platform to study synaptic communication between neuronal populations in vitro. We first show that Calcium Orange indicator is compatible in vitro with a commonly used Channelrhodopsi...

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Bibliographic Details
Main Authors: Renaud Renault, Nirit Sukenik, Stéphanie Descroix, Laurent Malaquin, Jean-Louis Viovy, Jean-Michel Peyrin, Samuel Bottani, Pascal Monceau, Elisha Moses, Maéva Vignes
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4406441?pdf=render

Internet

http://europepmc.org/articles/PMC4406441?pdf=render

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