Isolation of human cutaneous immune cells for single-cell RNA sequencing

Isolation of human cutaneous immune cells for single-cell RNA sequencing

Summary: Single-cell RNA sequencing (scRNA-seq) allows for high-resolution analysis of transcriptionally dysregulated cell subpopulations in inflammatory diseases. However, it can be challenging to properly isolate viable immune cells from human skin for scRNA-seq due to its barrier properties. Here...

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Bibliographic Details
Main Authors: Ashley A. Hailer, David Wu, Abdullah El Kurdi, Michelle Yuan, Raymond J. Cho, Jeffrey B. Cheng
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Cell Isolation
Flow Cytometry/Mass Cytometry
Immunology
Molecular Biology
RNAseq
Single Cell
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723001971

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http://www.sciencedirect.com/science/article/pii/S2666166723001971

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